PUBLICATION

Planar polarity pathway and Nance-Horan syndrome-like 1b have essential cell-autonomous functions in neuronal migration

Authors
Walsh, G.S., Grant, P.K., Morgan, J.A., and Moens, C.B.
ID
ZDB-PUB-140617-9
Date
2011
Source
Development (Cambridge, England)   138(14): 3033-3042 (Journal)
Registered Authors
Grant, Paul, Moens, Cecilia, Morgan, John, Walsh, Gregory
Keywords
none
MeSH Terms
  • Immunoprecipitation
  • Neurons/physiology*
  • In Situ Hybridization
  • Immunohistochemistry
  • Zebrafish Proteins/metabolism*
  • Plasmids/genetics
  • Signal Transduction/physiology*
  • Cell Movement/physiology*
  • Facial Nerve/cytology*
  • Animals
  • Membrane Proteins/metabolism*
  • Animals, Genetically Modified
  • Zebrafish/embryology*
  • Cells, Cultured
  • Mutagenesis
(all 15)
PubMed
21693519 Full text @ Development
Abstract

Components of the planar cell polarity (PCP) pathway are required for the caudal tangential migration of facial branchiomotor (FBM) neurons, but how PCP signaling regulates this migration is not understood. In a forward genetic screen, we identified a new gene, nhsl1b, required for FBM neuron migration. nhsl1b encodes a WAVE-homology domain-containing protein related to human Nance-Horan syndrome (NHS) protein and Drosophila GUK-holder (Gukh), which have been shown to interact with components of the WAVE regulatory complex that controls cytoskeletal dynamics and with the polarity protein Scribble, respectively. Nhsl1b localizes to FBM neuron membrane protrusions and interacts physically and genetically with Scrib to control FBM neuron migration. Using chimeric analysis, we show that FBM neurons have two modes of migration: one involving interactions between the neurons and their planar-polarized environment, and an alternative, collective mode involving interactions between the neurons themselves. We demonstrate that the first mode of migration requires the cell-autonomous functions of Nhsl1b and the PCP components Scrib and Vangl2 in addition to the non-autonomous functions of Scrib and Vangl2, which serve to polarize the epithelial cells in the environment of the migrating neurons. These results define a role for Nhsl1b as a neuronal effector of PCP signaling and indicate that proper FBM neuron migration is directly controlled by PCP signaling between the epithelium and the migrating neurons.

Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fh1TgTransgenic Insertion
    fh131
      Point Mutation
      fh280
        Point Mutation
        fh281
          Point Mutation
          m209
            Point Mutation
            rw0TgTransgenic Insertion
              rw468
                Point Mutation
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                1 - 7 of 7
                Show
                Fish
                Antibodies
                Name Type Antigen Genes Isotypes Host Organism
                Ab1-arl13bpolyclonalRabbit
                Ab1-nhsl1bpolyclonalRabbit
                Ab2-islmonoclonalIgG2bMouse
                Ab2-tjp1polyclonalRabbit
                1 - 4 of 4
                Show
                Orthology
                Gene Orthology
                nhsl1a
                nhsl1b
                1 - 2 of 2
                Show
                Engineered Foreign Genes
                Marker Marker Type Name
                GFPEFGGFP
                mRFPEFGmRFP
                1 - 2 of 2
                Show
                Mapping
                No data available