PUBLICATION

A Pou5f1/Oct4 dependent Klf2a, Klf2b, and Klf17 regulatory sub-network contributes to EVL and ectoderm development during zebrafish embryogenesis

Authors
Kotkamp, K., Mössner, R., Allen, A., Onichtchouk, D., and Driever, W.
ID
ZDB-PUB-131218-14
Date
2014
Source
Developmental Biology   385(2): 433-47 (Journal)
Registered Authors
Driever, Wolfgang
Keywords
Blastula, Ectoderm, Enveloping layer, Gastrulation, Gene regulatory network, Germ layers, Krüppel-like factors, Oct4, Pou5f1, Transcriptional regulation, Zebrafish
Datasets
GEO:GSE45011, GEO:GSE44982, GEO:GSE45013, GEO:GSE45012, GEO:GSE45010
MeSH Terms
  • Animals
  • Zebrafish/embryology*
  • Octamer Transcription Factor-3/physiology*
  • Kruppel-Like Transcription Factors/genetics*
  • Blastula/embryology*
  • Blastula/metabolism
  • Signal Transduction
  • Gene Regulatory Networks*
  • Ectoderm/embryology*
  • Ectoderm/metabolism
  • Zebrafish Proteins/physiology*
  • Bone Morphogenetic Proteins/metabolism
(all 12)
PubMed
24211655 Full text @ Dev. Biol.
Abstract

In mammalian ES cells, the transcription factors Klf4 and Klf2 contribute to maintenance of pluripotency and self-renewal and are regulated by Pou5f1/Oct4. In the early zebrafish embryo Pou5f1/Oct4 is necessary for expression of three Klf2/4 family members, klf2a, klf2b and klf17 (previously klf4b), similar to the regulation reported for mammalian ES cells. In this study, we analyzed blastula and gastrula stage Klf regulatory networks and their influence on zebrafish embryonic patterning. We show that Pou5f1 acts in combination with region-specific factors to activate klf2a, klf2b, and klf17 in the superficial cell layer of the embryo. In addition, Pou5f1 acts together with the BMP signaling pathway to activate and maintain expression of klf2a and klf2b in a ventral ectodermal domain. We used microarray expression profiles of klf2a, klf2b and klf17 knockdown and overexpression embryos to identify Klf target genes, which reveals that Klfs participate in specification of the extraembryonic enveloping layer (EVL). We discuss mechanistic implications of simultaneous activation of transcriptional targets by ubiquitous, like Pou5f1, and region-specific inducers, emerging as a common regulatory motif in early development.

Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m793
    Point Mutation
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    klf2aMO1-klf2aMRPHLNO
    klf2bMO1-klf2bMRPHLNO
    klf17MO1-klf17MRPHLNO
    1 - 3 of 3
    Show
    Fish
    Antibodies
    No data available
    Orthology
    Gene Orthology
    klf17
    1 - 1 of 1
    Show
    Engineered Foreign Genes
    No data available
    Mapping
    No data available