Gene
pou5f3
- ID
- ZDB-GENE-980526-485
- Name
- POU domain, class 5, transcription factor 3
- Symbol
- pou5f3 Nomenclature History
- Previous Names
- Type
- protein_coding_gene
- Location
- Chr: 21 Mapping Details/Browsers
- Description
- Enables several functions, including DNA-binding transcription factor activity; chromatin binding activity; and protein heterodimerization activity. Involved in nucleosome organization. Acts upstream of or within several processes, including brain development; embryonic morphogenesis; and regulation of gene expression. Predicted to be located in nucleus. Is expressed in several structures, including blastoderm; blastodisc; ectoderm; gonad; and hindbrain neural keel. Human ortholog(s) of this gene implicated in embryonal carcinoma and seminoma. Orthologous to human POU5F1 (POU class 5 homeobox 1); POU5F1B (POU class 5 homeobox 1B); and POU5F2 (POU domain class 5, transcription factor 2).
- Genome Resources
- Note
-
The gene name was updated on May 20, 2013 to reflect the evolutionary relationship of this gene with the mammalian POU domain, class 5 genes. The data suggests that there is not a true ortholog in human and mouse for this zebrafish gene. The synteny, amino acid alignment and phylogenetic trees suggest that a duplication event occurred in the last common ancestor of extant cartilaginous fishes and bony fishes resulting in 2 POU5 genes. Subsequently, the bony fishes, marsupials and birds maintained pou5f3, but lost pou5f1 which was maintained in eutherians and marsupials. This gene is an apparent ortholog (AA, CL) with the opposum, xenopus and chicken Pou5f3 genes.
- Comparative Information
-
- All Expression Data
- 68 figures from 37 publications
- Cross-Species Comparison
- High Throughput Data
- Thisse Expression Data
-
- cb197 (17 images)
Wild Type Expression Summary
- All Phenotype Data
- 63 figures from 23 publications
- Cross-Species Comparison
- Alliance
Phenotype Summary
Mutations
| Allele | Type | Localization | Consequence | Mutagen | Supplier |
|---|---|---|---|---|---|
| e68 | unknown | Unknown | Unknown | ENU | |
| e713 | Allele with one point mutation | Unknown | Missense | ENU | |
| hi349Tg | Transgenic insertion | Exon 3 | Unknown | DNA | |
| hi1757Tg | Transgenic insertion | Unknown | Unknown | DNA | |
| hi1940Tg | Transgenic insertion | Exon 1 | Unknown | DNA | |
| ihb137 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| ihb138 | Allele with one deletion | Unknown | Unknown | CRISPR | |
| ihb893 | Allele with one deletion | Exon 1 | Unknown | CRISPR | |
| ihb934 | Allele with one deletion | Exon 1 | Unknown | CRISPR | |
| ihb935 | Allele with one deletion | Exon 1 | Unknown | CRISPR |
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Human Disease
Domain, Family, and Site Summary
Domain Details Per Protein
| Protein | Additional Resources | Length | Homedomain-like superfamily | Homeobox, conserved site | Homeodomain | Lambda repressor-like, DNA-binding domain superfamily | POU domain | POU domain transcription factor | POU-specific domain |
|---|---|---|---|---|---|---|---|---|---|
| UniProtKB:Q90270 | InterPro | 472 | |||||||
| UniProtKB:A0A8M2B807 | InterPro | 471 | |||||||
| UniProtKB:A0A8M2B889 | InterPro | 399 | |||||||
| UniProtKB:A0A0R4I9C7 | InterPro | 472 | |||||||
| UniProtKB:A0AB32TCY0 | InterPro | 398 |
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| Type | Name | Annotation Method | Has Havana Data | Length (nt) | Analysis |
|---|---|---|---|---|---|
| mRNA |
pou5f3-201
(1)
|
Ensembl | 2,476 nt |
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Interactions and Pathways
No data available
| Name | Type | Antigen Genes | Isotype | Host Organism | Assay | Source | Citations |
|---|---|---|---|---|---|---|---|
| Ab3-pou5f3 | polyclonal | Rabbit |
|
1 | |||
| Ab2-pou5f3 | polyclonal | Rabbit |
|
1 |
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Plasmids
No data available
| Construct | Regulatory Region | Coding Sequence | Species | Tg Lines | Citations |
|---|---|---|---|---|---|
| Tg(-6.5pou5f1:EGFP) |
|
| 1 | (4) | |
| Tg(hsp70l:Dme.En-pou5f1) |
|
| 1 | (7) |
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| Relationship | Marker Type | Marker | Accession Numbers | Citations |
|---|---|---|---|---|
| Contained in | BAC | CH73-205E12 | ZFIN Curated Data | |
| Contains | SNP | rs3729086 | ZFIN Curated Data | |
| Encodes | EST | cb197 | Thisse et al., 2001 | |
| Encodes | EST | fd18d06 | ||
| Encodes | STS | chunp6868 | ||
| Encodes | cDNA | MGC:55202 | ZFIN Curated Data |
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| Type | Accession # | Sequence | Length (nt/aa) | Analysis |
|---|---|---|---|---|
| RNA | RefSeq:NM_131112 (1) | 2425 nt | ||
| Genomic | GenBank:BX649363 (1) | 196451 nt | ||
| Polypeptide | UniProtKB:A0A0R4I9C7 (1) | 472 aa |
No data available
- Ohyanagi, T., Tokizaki, H., Sato, T., Tsuruoka, M., Yamasu, K. (2025) Genetic Mechanism That Defines the Characteristic Neurogenesis Patterns in the Neural Plate Using Hes/her Genes During Early Vertebrate Development. Genesis (New York, N.Y. : 2000). 63:e70015e70015
- Zebrafish Nomenclature Committee (2025) Nomenclature Data Curation (2025). Nomenclature Committee Submission.
- Maekawa, M., Saito, S., Isobe, D., Takemoto, K., Miura, Y., Dobashi, Y., Yamasu, K. (2024) The Oct4-related PouV gene, pou5f3, mediates isthmus development in zebrafish by directly and dynamically regulating pax2a. Cells & development. 179:203933
- Colella, M., Cuomo, D., Nittoli, V., Amoresano, A., Porciello, A., Reale, C., Roberto, L., Russo, F., Russo, N.A., De Felice, M., Mallardo, M., Ambrosino, C. (2023) A Cross-Species Analysis Reveals Dysthyroidism of the Ovaries as a Common Trait of Premature Ovarian Aging. International Journal of Molecular Sciences. 24(3):
- Riesle, A.J., Gao, M., Rosenblatt, M., Hermes, J., Hass, H., Gebhard, A., Veil, M., Grüning, B., Timmer, J., Onichtchouk, D. (2023) Activator-blocker model of transcriptional regulation by pioneer-like factors. Nature communications. 14:56775677
- Sugitani, K., Mokuya, T., Homma, S., Maeda, M., Konno, A., Ogai, K. (2023) Specific Activation of Yamanaka Factors via HSF1 Signaling in the Early Stage of Zebrafish Optic Nerve Regeneration. International Journal of Molecular Sciences. 24(4):
- Wang, X., Zhu, J., Wang, H., Deng, W., Jiao, S., Wang, Y., He, M., Zhang, F., Liu, T., Hao, Y., Ye, D., Sun, Y. (2023) Induced formation of primordial germ cells from zebrafish blastomeres by germplasm factors. Nature communications. 14:79187918
- Yuikawa, T., Sato, T., Ikeda, M., Tsuruoka, M., Yasuda, K., Sato, Y., Nasu, K., Yamasu, K. (2023) Elongation of the developing spinal cord is driven by Oct4-type transcription factor-mediated regulation of retinoic acid signaling in zebrafish embryos. Developmental Dynamics : an official publication of the American Association of Anatomists. 253(4):404-422
- Zuppo, D.A., Missinato, M.A., Santana-Santos, L., Li, G., Benos, P.V., Tsang, M. (2023) Foxm1 regulates cardiomyocyte proliferation in adult zebrafish after cardiac injury. Development (Cambridge, England). 150(6):
- Doretto, L.B., Butzge, A.J., Nakajima, R.T., Martinez, E.R.M., de Souza, B.M., Rodrigues, M.D.S., Rosa, I.F., Ricci, J.M.B., Tovo-Neto, A., Costa, D.F., Malafaia, G., Shao, C., Nóbrega, R.H. (2022) Gdnf Acts as a Germ Cell-Derived Growth Factor and Regulates the Zebrafish Germ Stem Cell Niche in Autocrine- and Paracrine-Dependent Manners. Cells. 11(8):
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