PUBLICATION

Pou5f1-dependent EGF expression controls e-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements

Authors
Song, S., Eckerle, S., Onichtchouk, D., Marrs, J.A., Nitschke, R., and Driever, W.
ID
ZDB-PUB-130403-18
Date
2013
Source
Developmental Cell   24(5): 486-501 (Journal)
Registered Authors
Driever, Wolfgang, Marrs, James A.
Keywords
none
MeSH Terms
  • rab5 GTP-Binding Proteins/genetics
  • rab5 GTP-Binding Proteins/metabolism
  • Animals
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Cadherins/genetics
  • Cadherins/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Gastrulation/physiology*
  • Real-Time Polymerase Chain Reaction
  • Epidermal Growth Factor/metabolism*
  • Cell Adhesion/physiology*
  • Endocytosis/physiology*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Immunoenzyme Techniques
  • Gene Expression Regulation, Developmental
  • RNA, Messenger/genetics
  • Octamer Transcription Factor-3/genetics
  • Octamer Transcription Factor-3/metabolism*
  • Blotting, Western
  • Cell Movement/physiology
  • Genes, erbB-1
(all 27)
PubMed
23484854 Full text @ Dev. Cell
Abstract

Initiation of motile cell behavior in embryonic development occurs during late blastula stages when gastrulation begins. At this stage, the strong adhesion of blastomeres has to be modulated to enable dynamic behavior, similar to epithelial-to-mesenchymal transitions. We show that, in zebrafish maternal and zygotic (MZ)spg embryos mutant for the stem cell transcription factor Pou5f1/Oct4, which are severely delayed in the epiboly gastrulation movement, all blastomeres are defective in E-cadherin (E-cad) endosomal trafficking, and E-cad accumulates at the plasma membrane. We find that Pou5f1-dependent control of EGF expression regulates endosomal E-cad trafficking. EGF receptor may act via modulation of p120 activity. Loss of E-cad dynamics reduces cohesion of cells in reaggregation assays. Quantitative analysis of cell behavior indicates that dynamic E-cad endosomal trafficking is required for epiboly cell movements. We hypothesize that dynamic control of E-cad trafficking is essential to effectively generate new adhesion sites when cells move relative to each other.

Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
No data available
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m793
    Point Mutation
    m1032TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      rab5cMO1-rab5cMRPHLNO
      1 - 1 of 1
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      Fish
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      AB1-cdh1polyclonalRabbit
      Ab1-ctnnbmonoclonal
        IgG1Mouse
        Ab1-ctnndmonoclonal
          IgG1Mouse
          Ab1-rab4polyclonal
            IgGRabbit
            Ab2-cdh1monoclonal
              IgG2aMouse
              1 - 5 of 5
              Show
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              GFPEFGGFP
              1 - 1 of 1
              Show
              Mapping
              No data available