PUBLICATION

The interaction of epithelial Ihha and mesenchymal Fgf10 in zebrafish esophageal and swimbladder development

Authors
Korzh, S., Winata, C.L., Zheng, W., Yang, S., Yin, A., Ingham, P., Korzh, V., and Gong, Z.
ID
ZDB-PUB-110921-30
Date
2011
Source
Developmental Biology   359(2): 262-76 (Journal)
Registered Authors
Gong, Zhiyuan, Ingham, Philip, Korzh, Svitlana, Winata, Cecilia Lanny, Yang, Shulan, Yin, Ao
Keywords
ihha, gastrointestinal tract development, esophagus, swimbladder, epithelium, mesenchyme, fgf10
MeSH Terms
  • Gastrointestinal Tract/cytology
  • Gastrointestinal Tract/embryology
  • Gastrointestinal Tract/metabolism
  • Cell Proliferation
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Microscopy, Confocal
  • Animals
  • Air Sacs/embryology
  • Air Sacs/metabolism
  • Esophagus/embryology
  • Esophagus/metabolism
  • Gene Expression Regulation, Developmental
  • Female
  • Signal Transduction/genetics
  • Protein Binding
  • Fibroblast Growth Factor 10/genetics
  • Fibroblast Growth Factor 10/metabolism*
  • Receptors, Cell Surface/genetics
  • Receptors, Cell Surface/metabolism
  • In Situ Hybridization
  • Gene Knockdown Techniques
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Hedgehog Proteins/genetics
  • Hedgehog Proteins/metabolism*
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals, Genetically Modified
  • Epithelium/embryology
  • Epithelium/metabolism
  • Male
  • Mutation
(all 40)
PubMed
21925490 Full text @ Dev. Biol.
Abstract
Developmental patterning and growth of the vertebrate digestive and respiratory tracts requires interactions between the epithelial endoderm and adjacent mesoderm. The esophagus is a specialized structure that connects the digestive and respiratory systems and its normal development is critical for both. Shh signaling from the epithelium regulates related aspects of mammalian and zebrafish digestive organ development and has a prominent effect on esophageal morphogenesis. The mechanisms underlying esophageal malformations, however, are poorly understood. Here, we show that zebrafish Ihha signaling from the epithelium acting in parallel, but independently of Shh, controls epithelial and mesenchymal cell proliferation and differentiation of smooth muscles and neurons in the gut and swimbladder. In zebrafish ihha mutants, the esophageal and swimbladder epithelium is dysmorphic, and expression of fgf10 in adjacent mesenchymal cells is affected. Analysis of the development of the esophagus and swimbladder in fgf10 mutant daedalus (dae) and compound dae/ihha mutants shows that the Ihha-Fgf10 regulatory interaction is realized through a signaling feedback loop between the Ihha-expressing epithelium and Fgf10-expressing mesenchyme. Disruption of this loop further affects the esophageal and swimbladder epithelium in ihha mutants, and Ihha acts in parallel to but independently of Shha in this process. These findings contribute to the understanding of epithelial-mesenchymal interactions and highlight an interaction between Hh and Fgf signaling pathways during esophagus and swimbladder development.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    Point Mutation
    hu2131
      Point Mutation
      sqet332EtTransgenic Insertion
        t4
          Deficiency
          tbvbo
            Point Mutation
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            ihhaMO1-ihhaMRPHLNO
            tp53MO4-tp53MRPHLNO
            1 - 2 of 2
            Show
            Fish
            Antibodies
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            1 - 1 of 1
            Show
            Mapping
            No data available