PUBLICATION

Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart

Authors
de Pater, E., Clijsters, L., Marques, S.R., Lin, Y.F., Garavito-Aguilar, Z.V., Yelon, D., and Bakkers, J.
ID
ZDB-PUB-090429-10
Date
2009
Source
Development (Cambridge, England)   136(10): 1633-1641 (Journal)
Registered Authors
Bakkers, Jeroen, Yelon, Deborah
Keywords
Fgf, Differentiation, Heart, Islet1, Zebrafish
MeSH Terms
  • Cell Proliferation
  • Heart/embryology*
  • Heart/physiology
  • Animals
  • Fibroblast Growth Factors/physiology
  • Signal Transduction/physiology
  • Zebrafish/embryology*
  • Zebrafish/physiology
  • Myocytes, Cardiac/cytology*
  • Myocytes, Cardiac/physiology
  • Cell Differentiation/physiology*
(all 11)
PubMed
19395641 Full text @ Development
Abstract
Amongst animal species, there is enormous variation in the size and complexity of the heart, ranging from the simple one-chambered heart of Ciona intestinalis to the complex four-chambered heart of lunged animals. To address possible mechanisms for the evolutionary adaptation of heart size, we studied how growth of the simple two-chambered heart in zebrafish is regulated. Our data show that the embryonic zebrafish heart tube grows by a substantial increase in cardiomyocyte number. Augmented cardiomyocyte differentiation, as opposed to proliferation, is responsible for the observed growth. By using transgenic assays to monitor developmental timing, we visualized for the first time the dynamics of cardiomyocyte differentiation in a vertebrate embryo. Our data identify two previously unrecognized phases of cardiomyocyte differentiation separated in time, space and regulation. During the initial phase, a continuous wave of cardiomyocyte differentiation begins in the ventricle, ends in the atrium, and requires Islet1 for its completion. In the later phase, new cardiomyocytes are added to the arterial pole, and this process requires Fgf signaling. Thus, two separate processes of cardiomyocyte differentiation independently regulate growth of the zebrafish heart. Together, our data support a model in which modified regulation of these distinct phases of cardiomyocyte differentiation has been responsible for the changes in heart size and morphology among vertebrate species.
Genes / Markers
Figures
Figure Gallery (14 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
f2Tg/+; twu26Tg/+chemical treatment: SU5402Long-pec
f2Tg/+; twu26Tg/+ + MO2-fgf8astandard conditionsLong-pec
f2Tg/+; twu26Tg/+ + MO2-fgf8astandard conditionsLong-pec
isl1asa29/sa29standard conditionsPrim-5
isl1asa29/sa29standard conditionsPrim-5
isl1asa29/sa29standard conditionsPrim-5
isl1asa29/sa29standard conditionsLong-pec
isl1asa29/sa29standard conditionsLong-pec
isl1asa29/sa29standard conditionsProtruding-mouth
isl1asa29/sa29standard conditionsProtruding-mouth
1 - 10 of 13
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
f2TgTransgenic Insertion
    sa29
      		Point Mutation
      sd22TgTransgenic Insertion
        twu26TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          fgf8aMO2-fgf8aMRPHLNO
          isl1aMO2-isl1aMRPHLNO
          isl1aMO3-isl1aMRPHLNO
          tp53MO4-tp53MRPHLNO
          1 - 4 of 4
          Show
          Fish
          Fish
          f2Tg/+
          f2Tg/+; twu26Tg/+
          f2Tg/+; twu26Tg/+ + MO2-fgf8a
          isl1asa29/sa29
          isl1asa29/sa29; f2Tg/+; twu26Tg/+
          sd22Tg
          twu26Tg/+
          WT
          1 - 8 of 8
          Show
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          DsRed2EFGDsRed2
          EGFPEFGEGFP
          KaedeEFGKaede
          1 - 3 of 3
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          Mapping
          No data available
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          Citation
          de Pater, E., Clijsters, L., Marques, S.R., Lin, Y.F., Garavito-Aguilar, Z.V., Yelon, D., and Bakkers, J. (2009) Distinct phases of cardiomyocyte differentiation regulate growth of the zebrafish heart. Development (Cambridge, England). 136(10):1633-1641.