PUBLICATION

Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei

Authors

Roussigné, M., Bianco, I.H., Wilson, S.W., and Blader, P.

ID

ZDB-PUB-090417-1

Date

2009

Source

Development (Cambridge, England) 136(9): 1549-1557 (Journal)

Registered Authors

Bianco, Isaac, Blader, Patrick, Roussigné, Myriam, Wilson, Steve

Keywords

Nodal signalling, Asymmetry, Habenular nuclei, Neurogenesis, Zebrafish

MeSH Terms

Body Patterning*
Nodal Protein/genetics
Nodal Protein/metabolism*
Animals
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
Habenula/embryology*
Habenula/metabolism*
Gene Expression Regulation, Developmental
Receptors, CXCR4/genetics
Receptors, CXCR4/metabolism
Neurogenesis*
Signal Transduction

(all 14)

PubMed

19363156 Full text @ Development

Abstract

The habenulae are evolutionarily conserved bilateral nuclei in the epithalamus that relay input from the forebrain to the ventral midbrain. In zebrafish, the habenulae display left-right (L/R) asymmetries in gene expression and axonal projections. The elaboration of habenular asymmetries requires the presence of a second asymmetric structure, the parapineal, the laterality of which is biased by unilateral Nodal signalling. Here we show that neurons are present earlier in the left habenula than in the right, but, in contrast to other habenular asymmetry phenotypes, this asymmetry in neurogenesis is not dependent on the parapineal. Embryos in which the L/R asymmetry in Nodal signalling is abolished display symmetric neurogenesis, revealing a requirement for this pathway in asymmetrically biasing neurogenesis. Our results provide evidence of a direct requirement for unilateral Nodal activity in establishing an asymmetry per se, rather than solely in biasing its laterality.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
cxcr4b	knu3Tg	standard conditions	Prim-5	epiphysis	ISH	Fig. 3
cxcr4b	knu3Tg	standard conditions	Prim-15	epiphysis	ISH	Fig. 3
cxcr4b	knu3Tg	standard conditions	Prim-25	epiphysis	ISH	Fig. 3
cxcr4b	knu3Tg	standard conditions	Prim-25	epithalamus	ISH	Fig. 3
cxcr4b	knu3Tg	standard conditions	Prim-15	epithalamus	ISH	Fig. 3
cxcr4b	knu3Tg	standard conditions	Prim-5	epithalamus	ISH	Fig. 3
cxcr4b	knu3Tg	standard conditions	Long-pec	habenula	ISH	Fig. 1
cxcr4b	knu3Tg	standard conditions	Long-pec	habenula	ISH	Fig. 2
cxcr4b	sb1Tg	standard conditions	Long-pec	habenula	ISH	Fig. 2
cxcr4b	WT	standard conditions	Prim-25	epithalamus	ISH	Fig. 1

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	cold exposure	Prim-15 to Prim-25	habenula determination of left/right symmetry disrupted, abnormal	Fig. 5
WT + MO1-spaw	standard conditions	20-25 somites to 26+ somites	epithalamus determination of left/right symmetry disrupted, abnormal	Fig. T1
WT + MO1-spaw	standard conditions	Prim-15 to Prim-25	habenula determination of left/right symmetry disrupted, abnormal	Fig. 5
WT + MO1-spaw	standard conditions	Prim-15 to Prim-25	habenula neurogenesis process quality, abnormal	Fig. 5
WT + MO1-spaw	standard conditions	Prim-15 to Prim-25	nodal signaling pathway disrupted, abnormal	Fig. 5
WT + MO1-tbxta	standard conditions	14-19 somites to 20-25 somites	epithalamus determination of left/right symmetry disrupted, abnormal	Fig. T1
WT + MO1-tbxta	standard conditions	Prim-15 to Prim-25	habenula determination of left/right symmetry disrupted, abnormal	Fig. 5
WT + MO1-tbxta	standard conditions	Prim-15 to Prim-25	habenula neurogenesis process quality, abnormal	Fig. 5
WT + MO1-tbxta	standard conditions	Prim-15 to Prim-25	nodal signaling pathway disrupted, abnormal	Fig. 5
knu3Tg	chemical treatment: pharmaceutical	Prim-15	habenula determination of left/right symmetry disrupted, abnormal	Fig. 6

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Mutations / Transgenics

Allele	Construct	Type
knu3Tg	Tg(elavl3:EGFP)	Transgenic Insertion
sb1Tg	Tg(-8.4neurog1:GFP)	Transgenic Insertion
u711Tg	Tg(-1.6flh:GAP-EGFP)	Transgenic Insertion
zf15Tg	Tg(foxd3:GFP)	Transgenic Insertion

1 - 4 of 4

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
spaw	MO1-spaw	MRPHLNO
tbxta	MO1-tbxta	MRPHLNO

Fish

Fish
knu3Tg
sb1Tg
u711Tg
WT
WT + MO1-spaw
WT + MO1-tbxta
zf15Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GFP	EFG	GFP

Mapping

Roussigné et al., 2009 ZDB-PUB-090417-1 PMID:19363156

Nodal signalling imposes left-right asymmetry upon neurogenesis in the habenular nuclei

Roussigné et al., 2009

ZDB-PUB-090417-1
PMID:19363156