PUBLICATION

Lgl2 and E-cadherin act antagonistically to regulate hemidesmosome formation during epidermal development in zebrafish

Authors
Sonawane, M., Martin-Maischein, H., Schwarz, H., and Nüsslein-Volhard, C.
ID
ZDB-PUB-090310-9
Date
2009
Source
Development (Cambridge, England)   136(8): 1231-1240 (Journal)
Registered Authors
Nüsslein-Volhard, Christiane, Sonawane, Mahendra
Keywords
Lgl2 (Llgl2), E-cadherin (Cadherin 1), Hemidesmosome formation, Epidermis, Zebrafish
MeSH Terms
  • Animals
  • Cell Membrane/metabolism
  • Hemidesmosomes/genetics
  • Hemidesmosomes/metabolism*
  • Cadherins/metabolism*
  • Gene Expression Regulation, Developmental
  • Epidermis/cytology
  • Epidermis/growth & development*
  • Epidermis/metabolism*
  • Microscopy, Immunoelectron
  • Integrin alpha6/metabolism
  • beta Karyopherins/genetics
  • beta Karyopherins/metabolism*
  • Isoenzymes/metabolism
  • Cell Differentiation
  • Protein Kinase C/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism*
(all 19)
PubMed
19261700 Full text @ Development
Abstract
The integrity and homeostasis of the vertebrate epidermis depend on various cellular junctions. How these junctions are assembled during development and how their number is regulated remain largely unclear. Here, we address these issues by analysing the function of Lgl2, E-cadherin and atypical Protein kinase C (aPKC) in the formation of hemidesmosomes in the developing basal epidermis of zebrafish larvae. Previously, we have shown that a mutation in lgl2 (penner) prevents the formation of hemidesmosomes. Here we show that Lgl2 function is essential for mediating the targeting of Integrin alpha 6 (Itga6), a hemidesmosomal component, to the plasma membrane of basal epidermal cells. In addition, we show that whereas aPKClambda seems dispensable for the localisation of Itga6 during hemidesmosome formation, knockdown of E-cadherin function leads to an Lgl2-dependent increase in the localisation of Itga6. Thus, Lgl2 and E-cadherin act antagonistically to control the localisation of Itga6 during the formation of hemidesmosomes in the developing epidermis.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m567
    Point Mutation
    slc45a2_unspecified
      Unspecified
      to6
        Point Mutation
        zp5TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          cdh1MO3-cdh1MRPHLNO
          llgl2MO1-llgl2MRPHLNO
          prkciMO1-prkciMRPHLNO
          1 - 3 of 3
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          Fish
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          1 - 1 of 1
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          Mapping