PUBLICATION

Hedgehog signalling is required for cloacal development in the zebrafish embryo

Authors

Parkin, C.A., Allen, C.E., and Ingham, P.W.

ID

ZDB-PUB-090106-43

Date

2009

Source

The International journal of developmental biology 53(1): 45-57 (Journal)

Registered Authors

Allen, Claire, Ingham, Philip, Parkin, Caroline

Keywords

zebrafish, sonic hedgehog, gut, anorectal malformations, cloaca, stenosis, Adriamycin

MeSH Terms

Signal Transduction*/drug effects
Hedgehog Proteins/genetics
Hedgehog Proteins/metabolism*
Veratrum Alkaloids/pharmacology
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/metabolism*
Gene Expression Regulation, Developmental
Cloaca/cytology
Cloaca/embryology*
Cloaca/metabolism*
Mutation/genetics
Apoptosis
Animals
Animals, Genetically Modified
Transcription Factors/genetics
Transcription Factors/metabolism
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism

(all 19)

PubMed

19123126 Full text @ Int. J. Dev. Biol.

Abstract

The Hedgehog (Hh) family of signalling molecules is essential for a wide range of developmental processes. Mammalian studies have implicated the Hedgehog pathway in the aetiology of anorectal malformations (ARMs), relatively common congenital anomalies caused by failures in the development of the cloaca. In this study we demonstrate that Hh signalling is absolutely required for the formation of the zebrafish cloaca and that the severity of the posterior gut abnormalities induced by a reduction in Hh activity is dependent on the levels of Hh signal transduction. The complete loss of all Hh activity results in the most severe defects and the critical period for Hh activity is between 34 and 74 hours post fertilisation. Using a range of mutant genotypes that cause notochord and floorplate abnormalities, we show that the source of the Hh signals required for posterior gut formation is the endoderm and not the notochord, as previously postulated in mammalian models of ARMs. We show that Adriamycin, a drug known to cause ARMs in rat, but not chick embryos, has no effect on the development of the zebrafish gastrointestinal tract. These studies establish the zebrafish as a model for ARMs, and for the elucidation of other pathways involved in hindgut developmental processes.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
Df(Chr07)t4/t4	standard conditions	Prim-5 to Day 5	apoptotic process occurrence, normal	Fig. 6
Df(Chr07)t4/t4	standard conditions	Day 5	proctodeum atretic, abnormal	Fig. 3
Df(Chr07)t4/t4	standard conditions	Day 5	proctodeum malformed, abnormal	Fig. 3
ctd^{unspecified/unspecified}	standard conditions	Day 5	notochord undulate, abnormal	Fig. 8
ctd^{unspecified/unspecified}	standard conditions	Day 5	proctodeum open, normal	Fig. 8
ctd^{unspecified/unspecified}	standard conditions	Day 5	pronephric duct opening morphology, normal	Fig. 8
doc^{unspecified/unspecified}	standard conditions	Day 5	proctodeum open, normal	Fig. 8
gli2a^ty119/ty119	standard conditions	Day 5	proctodeum atretic, abnormal	Fig. 3
gli2a^ty119/ty119	standard conditions	Day 5	proctodeum malformed, abnormal	Fig. 3
ihha^{hu2131/hu2131}	standard conditions	Day 5	proctodeum morphology, normal	text only

1 - 10 of 22

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Mutations / Transgenics

Allele	Type	Affected Genomic Region
b104	Indel	tbx16
b577	Point Mutation	smo
b641	Point Mutation	smo
ctd_unspecified	Unspecified	ctd
doc_unspecified	Unspecified	doc
hu2131	Point Mutation	ihha
lama1_unspecified	Unspecified	lama1
lamc1_unspecified	Unspecified	lamc1
noto_unspecified	Unspecified	noto
t4	Deficiency	shha

1 - 10 of 15

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
ctd^{unspecified/unspecified}
Df(Chr07)t4/t4
doc^{unspecified/unspecified}
gli2a^ty119/ty119
ihha^{hu2131/hu2131}
lama1^{unspecified/unspecified}
lamc1^{unspecified/unspecified}
noto^{unspecified/unspecified}
smo^b577/b577
tbx16^{unspecified/unspecified}

1 - 10 of 12

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

Parkin et al., 2009 ZDB-PUB-090106-43 PMID:19123126

Hedgehog signalling is required for cloacal development in the zebrafish embryo

Parkin et al., 2009

ZDB-PUB-090106-43
PMID:19123126