PUBLICATION

Genetic determinants of hyaloid and retinal vasculature in zebrafish

Authors
Alvarez, Y., Cederlund, M.L., Cottell, D.C., Bill, B.R., Ekker, S.C., Torres-Vazquez, J., Weinstein, B.M., Hyde, D.R., Vihtelic, T.S., and Kennedy, B.N.
ID
ZDB-PUB-071023-7
Date
2007
Source
BMC Developmental Biology   7(1): 114 (Journal)
Registered Authors
Alvarez, Yolanda, Bill, Brent, Cederlund, Maria, Ekker, Stephen C., Hyde, David R., Kennedy, Breandan N., Torres-Vázquez, Jesús, Vihtelic, Thomas, Weinstein, Brant M.
Keywords
none
MeSH Terms
  • Animals
  • Receptors, Cell Surface/genetics
  • Contractile Proteins/genetics
  • Choroid/blood supply*
  • Choroid/growth & development
  • Species Specificity
  • Gene Expression Regulation, Developmental*
  • Retinal Neovascularization/genetics
  • Neovascularization, Physiologic/genetics
  • Retinal Vessels/growth & development*
  • Humans
  • Extracellular Matrix Proteins/genetics
  • Optic Disk/blood supply
  • Embryo, Nonmammalian
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
  • Zebrafish Proteins/genetics
  • Immunohistochemistry
(all 18)
PubMed
17937808 Full text @ BMC Dev. Biol.
Abstract
BACKGROUND: The retinal vasculature is a capillary network of blood vessels that nourishes the inner retina of most mammals. Developmental abnormalities or microvascular complications in the retinal vasculature result in severe human eye diseases that lead to blindness. To exploit the advantages of zebrafish for genetic, developmental and pharmacological studies of retinal vasculature, we characterised the intraocular vasculature in zebrafish. RESULTS: We show a detailed morphological and developmental analysis of the retinal blood supply in zebrafish. Similar to the transient hyaloid vasculature in mammalian embryos, vessels are first found attached to the zebrafish lens at 2.5 days post fertilisation. These vessels progressively lose contact with the lens and by 30 days post fertilisation adhere to the inner limiting membrane of the juvenile retina. Ultrastructure analysis shows these vessels to exhibit distinctive hallmarks of mammalian retinal vasculature. For example, smooth muscle actin-expressing pericytes are ensheathed by the basal lamina of the blood vessel, and vesicle vacuolar organelles (VVO), subcellular mediators of vessel-retinal nourishment, are present. Finally, we identify 9 genes with cell membrane, extracellular matrix and unknown identity that are necessary for zebrafish hyaloid and retinal vasculature development. CONCLUSIONS: Zebrafish have a retinal blood supply with a characteristic developmental and adult morphology. Abnormalities of these intraocular vessels are easily observed, enabling application of genetic and chemical approaches in zebrafish to identify molecular regulators of hyaloid and retinal vasculature in development and disease.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
cdiptnt9/nt9standard conditionsDay 6
dslnt8/nt8standard conditionsDay 5 to Days 7-13
dslnt8/nt8standard conditionsDay 5 to Days 7-13
mgfnt5/nt5standard conditionsDay 4
mgfnt5/nt5standard conditionsDay 4
mgfnt5/nt5standard conditionsDay 5
plxnd1fov01b/fov01bstandard conditionsDay 5
plxnd1fov01b/fov01bstandard conditionsDay 5
plxnd1fov01b/fov01bstandard conditionsAdult
plxnd1fov01b/fov01bstandard conditionsAdult
1 - 10 of 19
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b4
    		Insertion
    fov01b
      		Point Mutation
      mi2001TgTransgenic Insertion
        nt5
          		Unknown
          nt7
            		Point Mutation
            nt8
              		Unknown
              nt9
                		Point Mutation
                ti282a
                  		Point Mutation
                  ucd1TgTransgenic Insertion
                    y1TgTransgenic Insertion
                      1 - 10 of 10
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      hs6st2MO2-hs6st2MRPHLNO
                      mab21l2MO1-mab21l2MRPHLNO
                      mfap2MO1-mfap2MRPHLNO
                      sdc2MO1-sdc2MRPHLNO
                      tp53MO4-tp53MRPHLNO
                      1 - 5 of 5
                      Show
                      Fish
                      Fish
                      cdiptnt9/nt9
                      dslnt8/nt8
                      fgf8ati282a/ti282a
                      lama1nt7/nt7
                      mgfnt5/nt5
                      mi2001Tg
                      plxnd1fov01b/fov01b
                      slc45a2b4/b4
                      ucd1Tg
                      WT
                      1 - 10 of 14
                      Show
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GFPEFGGFP
                      1 - 2 of 2
                      Show
                      Mapping
                      No data available
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                      Citation
                      Alvarez, Y., Cederlund, M.L., Cottell, D.C., Bill, B.R., Ekker, S.C., Torres-Vazquez, J., Weinstein, B.M., Hyde, D.R., Vihtelic, T.S., and Kennedy, B.N. (2007) Genetic determinants of hyaloid and retinal vasculature in zebrafish. BMC Developmental Biology. 7(1):114.