PUBLICATION

FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation

Authors
Seiliez, I., Thisse, B., and Thisse, C.
ID
ZDB-PUB-051221-5
Date
2006
Source
Developmental Biology   290(1): 152-163 (Journal)
Registered Authors
Seiliez, Iban, Thisse, Bernard, Thisse, Christine
Keywords
Goosecoid; foxA3; foxA2; dkk1; frzb; Wnt8a; Nervous system patterning; Posteriorization; Antero-posterior axis; Zebrafish; Embryo; Blastula
MeSH Terms
  • Cell Transplantation
  • Blastula/metabolism
  • Nervous System/embryology*
  • Nervous System/metabolism
  • Zebrafish Proteins/metabolism*
  • Up-Regulation
  • Wnt Proteins/metabolism*
  • Molecular Sequence Data
  • Head/embryology
  • Head/physiology
  • Goosecoid Protein/metabolism*
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Hepatocyte Nuclear Factor 3-gamma/metabolism*
  • Body Patterning
  • Amino Acid Sequence
  • Cytoskeletal Proteins/metabolism*
  • Signal Transduction
(all 19)
PubMed
16364286 Full text @ Dev. Biol.
Abstract
Formation of the nervous system initially requires the acquisition of neural identity, which is achieved through the inhibition of epidermalizing factors. A regional patterning then takes place within the neural plate through the activity of caudalizing factors. These two processes are tightly regulated early in development by the dorsal organizer. Here, we show that, in zebrafish embryos, two transcription factors, FoxA3 and Goosecoid, coexpressed at the dorsal blastula margin, are required for the definition of anterior neural fate. Their inactivation results in deletions of anterior head structures associated with an increase of Wnt8 activity at the dorsal blastula margin. These phenotypes can be fully rescued by overexpression of Wnt inhibitors or by inactivation of wnt8a. Altogether, foxA3 and goosecoid cooperate to promote formation of anterior neural tissue by protecting, as early as blastula stage, presumptive anterior neural cells from an irreversible caudalization by the posteriorizing factor Wnt8a.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
Target Reagent Reagent Type
dkk1bMO1-dkk1bMRPHLNO
foxa2MO1-foxa2MRPHLNO
foxa3MO1-foxa3MRPHLNO
gscMO1-gscMRPHLNO
gscMO2-gscMRPHLNO
gscMO3-gscMRPHLNO
wnt8aMO1-wnt8aMRPHLNO
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Fish
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Engineered Foreign Genes
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Mapping
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