PUBLICATION

Fgf8 drives myogenic progression of a novel lateral fast muscle fibre population in zebrafish

Authors
Groves, J.A., Hammond, C.L., and Hughes, S.M.
ID
ZDB-PUB-050831-2
Date
2005
Source
Development (Cambridge, England)   132(19): 4211-4222 (Journal)
Registered Authors
Groves, Julie, Hammond, Chrissy, Hughes, Simon M.
Keywords
Fibroblast growth factor 8, Muscle, Zebrafish, Fast, Myod, Somite
MeSH Terms
  • Paired Box Transcription Factors/biosynthesis
  • Trans-Activators/physiology
  • Animals
  • Somites/physiology*
  • Signal Transduction
  • Embryo, Nonmammalian/metabolism
  • Myogenin/metabolism
  • Hedgehog Proteins
  • Body Patterning/physiology
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • MyoD Protein/biosynthesis
  • Fibroblast Growth Factor 8/physiology*
  • Zebrafish Proteins/biosynthesis
  • Cell Differentiation
  • Myogenic Regulatory Factor 5/biosynthesis
  • Myoblasts/metabolism
  • Myoblasts/physiology*
  • Muscle Fibers, Fast-Twitch/cytology
  • Muscle Fibers, Fast-Twitch/physiology*
(all 20)
PubMed
16120642 Full text @ Development
Abstract
Fibroblast growth factors (Fgfs) have long been implicated in regulating vertebrate skeletal muscle differentiation, but their precise role(s) in vivo remain unclear. Here, we show that Fgf8 signalling in the somite is required for myod expression and terminal differentiation of a subset of fast muscle cells in the zebrafish lateral somite. In the absence of Fgf8, lateral somite cells transiently express myf5 but fail to make muscle and remain in a dermomyotome-like state characterised by pax3 and meox expression. Slow muscle fibres form and commence normal migration in the absence of Fgf8, but fail to traverse the expanded undifferentiated lateral somite. The Fgf8-independent residual population of medial fast muscle fibres is not Hedgehog dependent. However, Fgf8-independent medial fast muscle precursors are lacking in floatinghead mutants, suggesting that they require another ventral midline-derived signal. We conclude that Fgf8 drives terminal differentiation of a specific population of lateral muscle precursor cells within the early somite.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
n1
    Small Deletion
    ti282a
      Point Mutation
      ty119
        Point Mutation
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        fgf8aMO3-fgf8aMRPHLNO
        1 - 1 of 1
        Show
        Fish
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available