PUBLICATION

Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification

Authors

Hernandez-Lagunas, L., Choi, I.F., Kaji, T., Simpson, P., Hershey, C., Zhou, Y., Zon, L., Mercola, M., and Artinger, K.B.

ID

ZDB-PUB-050202-1

Date

2005

Source

Developmental Biology 278(2): 347-357 (Journal)

Registered Authors

Artinger, Kristin Bruk, Choi, Irene, Hernandez, Laura, Kaji, Takao, Simpson, Pete, Zhou, Yi, Zon, Leonard I.

Keywords

Narrowminded; Neural crest; Rohon–Beard sensory neurons; Blimp-1

MeSH Terms

In Situ Hybridization
Basic Helix-Loop-Helix Transcription Factors
Animals
DNA Primers
Nuclear Proteins
Zebrafish Proteins/genetics*
Zinc Fingers
DNA-Binding Proteins
Repressor Proteins/genetics*
Neural Crest/physiology*
Neurons, Afferent/cytology*
Nerve Tissue Proteins/genetics*
Transcription Factors/genetics*
Chromosome Mapping
Restriction Mapping
Base Sequence
Embryo, Nonmammalian/physiology
Zebrafish/embryology*

(all 18)

PubMed

15680355 Full text @ Dev. Biol.

Abstract

Specification of both neural crest cells and Rohon-Beard (RB) sensory neurons involves a complex series of interactions between the neural and non-neural ectoderm. The molecular mechanisms directing this process are not well understood. The zebrafish narrowminded (nrd) mutation is unique, since it is one of two mutations in which defects are observed in both cell populations: it leads to a complete absence of RB neurons and a reduction in neural crest cells and their derivatives. Here, we show that nrd is a mutation in prdm1, a SET/zinc-finger domain transcription factor. A Morpholino-mediated depletion of prdm1 phenocopies the nrd mutation, and conversely overexpression of prdm1 mRNA rescues the nrd RB sensory neuron and neural crest phenotype. prdm1 is expressed at the border of the neural plate within the domain where neural crest cells and RB sensory neurons form. Analysis of prdm1 function by overexpression indicates that prdm1 functions to promote the cell fate specification of both neural crest cells and RB sensory neurons, most likely as a downstream effector of the BMP signaling pathway.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
crestin	prdm1a^m805/m805	standard conditions	14-19 somites	neural crest	ISH	Fig. 2
crestin	prdm1a^m805/m805	standard conditions	Prim-5	unspecified	ISH	Fig. 3
crestin	WT	standard conditions	14-19 somites	neural crest	ISH	Fig. 2
crestin	WT	standard conditions	Prim-5	neuroectoderm	ISH	Fig. 7
crestin	WT	standard conditions	Prim-5	unspecified	ISH	Fig. 3
crestin	WT + MO2-prdm1a	standard conditions	14-19 somites	neural crest	ISH	Fig. 2
elavl3	prdm1a^m805/m805	standard conditions	1-4 somites	primary neuron	ISH	Fig. 2
elavl3	WT	standard conditions	1-4 somites	primary neuron	ISH	Fig. 2
elavl3	WT	standard conditions	5-9 somites	Rohon-Beard neuron	ISH	Fig. 5
elavl3	WT	standard conditions	1-4 somites	Rohon-Beard neuron	ISH	Fig. 2

1 - 10 of 34

Show

Phenotype

Mutations / Transgenics

Allele	Type	Affected Genomic Region
bmp2b_unspecified	Unspecified	bmp2b
m805	Point Mutation	prdm1a
smad5_unspecified	Unspecified	smad5
ty68a	Point Mutation	bmp7a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
prdm1a	MO2-prdm1a	MRPHLNO

Fish

Fish
bmp2b^unspecified
bmp7a^ty68a/ty68a
prdm1a^m805/m805
smad5^unspecified
WT
WT + MO2-prdm1a

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Entity Type	Entity Symbol	Location
Feature	m805	Chr: 16 Details
SSLP	z6365	Chr: 16 Details
SSLP	z19602	Chr: 16 Details

Hernandez-Lagunas et al., 2005 ZDB-PUB-050202-1 PMID:15680355

Zebrafish narrowminded disrupts the transcription factor prdm1 and is required for neural crest and sensory neuron specification

Hernandez-Lagunas et al., 2005

ZDB-PUB-050202-1
PMID:15680355