PUBLICATION

The zebrafish colourless gene regulates development of non-ectomesenchymal neural crest derivatives

Authors

Kelsh, R.N. and Eisen, J.S.

ID

ZDB-PUB-000201-29

Date

2000

Source

Development (Cambridge, England) 127(3): 515-525 (Journal)

Registered Authors

Eisen, Judith S., Kelsh, Robert

Keywords

zebrafish; neural crest; cell fate; melanophore; xanthophore; iridophore; neuron; glia

MeSH Terms

Melanocytes/cytology
Zebrafish/embryology*
Zebrafish/genetics*
Morphogenesis
Humans
Neuroglia/cytology
Animals
Mesoderm/physiology
Neurons, Afferent/cytology
Cartilage, Articular/embryology
Neural Crest/cytology*
Neural Crest/physiology*
Face/embryology
Mosaicism
Enteric Nervous System/cytology
Enteric Nervous System/embryology
Neurons/cytology*
Neurons/physiology
Gene Deletion*
Pigmentation/genetics

(all 20)

PubMed

10631172 Full text @ Development

Abstract

Neural crest forms four major categories of derivatives: pigment cells, peripheral neurons, peripheral glia, and ectomesenchymal cells. Some early neural crest cells generate progeny of several fates. How specific cell fates become specified is still poorly understood. Here we show that zebrafish embryos with mutations in the colourless gene have severe defects in most crest-derived cell types, including pigment cells, neurons and specific glia. In contrast, craniofacial skeleton and medial fin mesenchyme are normal. These observations suggest that colourless has a key role in development of non-ectomesenchymal neural crest fates, but not in development of ectomesenchymal fates. Thus, the cls mutant phenotype reveals a segregation of ectomesenchymal and non-ectomesenchymal fates during zebrafish neural crest development. The combination of pigmentation and enteric nervous system defects makes colourless mutations a model for two human neurocristopathies, Waardenburg-Shah syndrome and Hirschsprung's disease.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
foxd3	sox10^t3/t3	standard conditions	Prim-5		cranium glial cell	ISH	Fig. 6
foxd3	WT	standard conditions	Prim-5		cranium glial cell	ISH	Fig. 6
gfap	sox10^t3/t3	standard conditions	Day 5	Not Detected	gut glial cell	IHC	Fig. 7
gfap	WT	standard conditions	Day 5		gut glial cell	IHC	Fig. 7

1 - 4 of 4

Show

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
sox10^t3/t3	standard conditions	Prim-5	melanoblast decreased amount, abnormal	Fig. 1
sox10^t3/t3	standard conditions	Prim-15	neural crest cell decreased amount, abnormal	Fig. 2
sox10^t3/t3	standard conditions	Day 5	enteric nervous system glial cell decreased amount, abnormal	Fig. 7
sox10^t3/t3	standard conditions	Days 7-13	dorsal root ganglion decreased amount, abnormal	Fig. 4
sox10^t3/t3	standard conditions	Days 7-13	dorsal root ganglion mislocalised, abnormal	Fig. 4
sox10^t3/t3	standard conditions	Days 7-13	enteric nervous system neuron decreased amount, abnormal	Fig. 3
sox10^tw11/tw11	standard conditions	Prim-5	melanoblast decreased amount, abnormal	Fig. 1
sox10^tw11/tw11	standard conditions	Prim-15	neural crest cell decreased amount, abnormal	Fig. 2
sox10^tw11/tw11	standard conditions	Day 5	enteric nervous system glial cell decreased amount, abnormal	Fig. 7
sox10^tw11/tw11	standard conditions	Days 7-13	dorsal root ganglion decreased amount, abnormal	Fig. 4

1 - 10 of 18

Show

Mutations / Transgenics

Allele	Type	Affected Genomic Region
b4	Insertion	slc45a2
t3	Insertion	sox10
tw2	Point Mutation	sox10
tw11	Point Mutation	sox10

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
slc45a2^b4/b4
sox10^t3/t3
sox10^tw2/tw2
sox10^tw11/tw11
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Kelsh et al., 2000 ZDB-PUB-000201-29 PMID:10631172

The zebrafish colourless gene regulates development of non-ectomesenchymal neural crest derivatives

Kelsh et al., 2000

ZDB-PUB-000201-29
PMID:10631172