|
The morpholino oligos (MO) for retinoschisin (RS1) homologs rs1a and rs1b were designed to block the translation of the mRNAs into proteins, and were developed alongside a control oligo (SC). A‐B: The mRNA sequence of the MO binding region was confirmed on Sanger sequencing for rs1a (A1) and rs1b (B1) to ensure perfect binding. A1‐B1: the first 50 nucleotides of the Danio Rerio rs1a (A1) and rs1b (B1) mRNA. The MO binding region is indicated in bold green text. Red indicates the start codon. A3‐B3: ‘'5’ to ‘'3’ sequence of the MO targeting rs1a (A3) and rs1b (B3) and their untargeted control oligos containing the same base pair ratios.
|