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rs1a and rs1b are expressed in the developing zebrafish eye from 48 hpf onwards. Gene expression was assessed at 1, 24, 48, 72, 96, and 120 hpf. A: The expression of reference gene elongating factor alpha (elfa) was consistent across all samples. Gene expressions of rs1a and rs1b were first detectable at 48 hpf and were detected throughout 120 hpf. B. Expression levels, as measured by RNA sequencing, of rs1a and rs1b. Hpf = hours post‐fertilization, B = blank,—RT = cDNA synthesis negative control without reverse transcriptase, ‐RNA = cDNA synthesis negative control without input RNA, PC = positive control with adult zebrafish eye cDNA, 1 = 1 hpf, 24 = 24 hpf, 72 = 72 hpf, 96 = 96 hpf, 120 = 120 hpf.
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