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More severe reduction in eye size in fzd5sgu1 homozygotes devoid of aamp function. (A) Phenotype of stable aampu601 mutant (bottom) 4 dpf larvae compared to wild type (top). Arrow indicates missing jaw cartilage. (B) Schematic of predicted protein product from aampu601 allele and representation of CRISPR guides used to target aamp. (C) Representative phenotypes of larvae derived from a fzd5sgu1/+ incross uninjected or injected with aamp guides. Genotype status detailed at the bottom right of each panel. (D) Eye size quantifications of the four genotypic groups shown in C, showing additive effect of fzd5 and aamp loss of function on eye size. Each data point represents one eye. A pairwise Tukey HSD post-hoc test revealed statistically significant eye size differences between the sibling and fzd5sgu1 groups (**P=0.00765794), and between the aampF0 sibling and aampF0;fzd5sgu1 groups (***P=0.00033637).
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