Zglp-1 inhibits the transcription of amh activated by Sf-1 in its zinc finger domain region. Aamh promoter activity test. The amh promoter demonstrated transcriptional activity in contrast to the control. B The regulatory relationships among Zglp-1, Sf-1, and the amh promoter determined by a dual-luciferase reporter gene assay. The activation of the amh promoter was suppressed when Zglp-1 and Sf-1 were co-transfected into HEK293T cells. C The functional domain of Zglp-1 that blocks the ability of Sf-1 to activate the amh promoter. a. The domain structure of zebrafish Zglp-1, as predicted by the SMART program, revealed two distinct sections within its amino acid sequence. In particular, amino acids 227–374 constituted the Zglp-1-ZnF region, which encompassed the zinc finger domain. Amino acids 1–226 formed the Zglp-1-N segment. b. The regulatory relationships among Zglp-1-N, Sf-1, and the amh promoter. c. The regulatory relationships among Zglp-1-ZnF, Sf-1, and the amh promoter. Zglp-1-ZnF, rather than Zglp-1-N, suppressed the ability of Sf-1 to activate the amh promoter. Data are presented as the mean ± SD. ***P < 0.001; ns, no statistical difference
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