Figure 5

Genetic inhibition of nuclear factor kappa B (NF-κB) in macrophages abolishes the hyperresistance of Samhd1-deficient zebrafish larvae to infection with Salmonella enterica serovar Typhimurium. (A) The zebrafish line expressing a dominant negative (DN) form of Nfkbiaa, Tg(UAS:dn-nfkbiaa), was outcrossed with the transgenic line Tg(mpeg1:gal4), and one-cell-stage embryos were injected with gRNA (samhd1 or STD)/Casp9 complexes and infected with STM as previously described. The average from two independent experiments with >80 larvae per group is shown. P-values were calculated using a long-range test. ns, not significant. ***p ≤ 0.001. (B) Model showing the restriction of NF-κB activation by Samhd1 in wild-type macrophages and the hyperresistance of Samhd-1deficient macrophages to STM infection through the induction of NF-κB activation.