Figure 1

Interferon (IFN) stimulation is required to trigger interferon-stimulated gene (ISG) upregulation in Samhd1-deficient zebrafish larvae. (A) Diagram indicating the protein domains. (B) Transcript levels of samhd1 by reverse transcription PCR (RT-PCR) in the head and tail of zebrafish larvae. The average from three independent experiments, each with 25 pooled heads or tails, is shown. (C) Transcript levels of isg15 by reverse transcription quantitative PCR (RT-qPCR) in the head of zebrafish larvae injected with gRNA (samhd1 or STD)/Cas9 complexes with or without 1 pg/egg of pcDNA-IFNphi3. Bars show the mean ± SEM of three independent experiments, each with 25 pooled heads. Data are represented as fold change from control (STD). P-values were calculated using Student’s t-test in (B) and using one-way ANOVA and Tukey’s multiple range test in (C). ns, not significant. *p ≤ 0.05.