Fig. 1

Confirm the sequence of the targeted genomic region of endogenous pdgfrb in the fish we raised (A) Screenshot of the Ensembl site showing the procedure for accessing the sequence of the zebrafish gene pdgfrb. (B) Screenshot of the Ensembl site showing the sequence of Cas9 target region for creating a knockin line targeting pdgfrb, encompassing a partial sequence of the last intron and last exon of pdgfrb. The last exon region is highlighted in red. (C) Screenshot presenting the Cas9 target within pdgfrb gene locus selected for creating knockin line. The stop codon and the primer binding sites are marked. (D) The sequencing results of the targeted region of pdgfrb compare to the reference sequence on Ensembl. REF represents the reference sequence of pdgfrb on Ensembl, seq represents the sequencing results for the target region of pdgfrb. The base marked in red represents the site of SNP.