FIGURE 7

Coilin N‐terminal mutation impairs the immune response. (A) qPCR analysis of IL‐1β, IL‐6, and IL‐4 mRNA expression in 3 dpf WT or ΔN mutants injected with PBS or LPS. Data represents 3 biological replicates with 2 technical repeats for a total N = 6. Error bars represent SD and black dots represent individual data points. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns = not significant for comparisons to WT PBS. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 for comparisons to WT LPS. (B) Illustration of the generation of COIL ΔN zebrafish expressing the neutrophil marker (mCherry: Lyz) and macrophage marker (eGFP: Mpeg). (C) Representative images of neutrophil accumulation around the yolk sac following injection of PBS or LPS in 3 dpf COIL ΔN1 lineage zebrafish. Arrows indicate neutrophils at the yolk sac that were applied to counts. (+) indicates a normal COIL allele and (−) indicates a ΔN COIL allele. (D) Histogram displaying the average number of neutrophils per yolk sac following injection of PBS or LPS in 3 dpf COIL ΔN1 lineage zebrafish. Error bars represent SD and black dots represent individual data points. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns = not significant for comparisons to (+/+) PBS. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 for comparisons to (+/+) LPS.