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Fig. 2

ID

ZDB-FIG-250415-9

Publication

Tian et al., 2025 - gdf11 is required for pronephros/cloaca development through targeting TGF-β signaling

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Fig. 2

gdf11 is essential for pronephric tubule and cloaca organogenesis. (A) Hematoxylin-Eosin stained sections of WT and gdf11 mutant larvae at the level of the glomerulus and proximal tubules at 48 hpf. Scale bar: 50 μm. (B) The expression of cdh17 in WT and gdf11 mutants under Tg (cdh17-dsRed) background. Scale bar: 200 μm. (C) In situ hybridization of nephrin (left) and wt1a (right) in wild-type and gdf11 mutant embryos at 48 hpf. (D) WISH analysis in WT and gdf11 mutants at 48 hpf for slc4a4, trpm7, and slc12a3 probes. (E) H&E staining of the pronephric duct and cloaca region in WT and gdf11 mutants at 48 hpf. Scale bar: 200 μm. (F) In situ hybridization with pax2a in WT and gdf11 mutants at 24 hpf. (G) Confocal images showing cilia specification in WT and mutant embryos at 24 hpf and 48 hpf. Scale bar: 50 μm. (H) Comparative in situ hybridization of cdh17 and pax2a expression in WT and gdf11 mutants at 10 ss. (I) WISH analysis for evx1 and prdm1 in WT and gdf11 mutants from 24 hpf to 72 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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