FIGURE

Fig. 3

ID

ZDB-FIG-250415-10

Publication

Tian et al., 2025 - gdf11 is required for pronephros/cloaca development through targeting TGF-β signaling

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Fig. 3

gdf11 knockdown disrupts pronephros and cloaca organogenesis. (A) Schematic representation of the plasmid used to test gdf11 MO efficiency. Scale bar: 500 μm. (B) Western blot showing a significant reduction of Gdf11 protein levels in gdf11 morphants. (C) Live imaging of control and gdf11 morphants, with or without gdf11 mRNA injection, from 36 hpf to 72 hpf. Scale bar: 300 μm. (D) Control and gdf11 morphant embryos harvested at 48 hpf were subjected to WISH for nephrin and wt1a expression. (E) ISH analysis using segment-specific probes for the pronephric tubules in control and gdf11 morphants at 48 hpf. (F) Lateral views of evx1 expression in control and gdf11 morphants from 24 hpf to 72 hpf.

Expression Data

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Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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