FIGURE

Fig. 8

ID

ZDB-FIG-250311-83

Publication

Patra et al., 2025 - Nephronectin Is Required for Vascularization in Zebrafish and Sufficient to Promote Mammalian Vessel-Like Structures in Hydrogels for Tissue Engineering

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Fig. 8

Nephronectin promotes HUVEC migration, tube formation, and stabilization in vitro. A, Cell migration assay: representative brightfield images of wound assays 1 and 6 hours after scratching. Cells were cultured on gelatin‐ (control) or mr‐NPNT‐coated 24‐well cell culture plates. B, Quantitative analysis of cell migration (n=4). The mean value of the control was considered 100% in each case. C, Tube formation assay: representative brightfield images of HUVECs cultured on Matrigel with BSA (10 μg/mL, control) or mr‐NPNT (10 μg/mL) at 4 and 22 hours after seeding. D–F, Quantitative analysis of tube branching (D), and tube stability (E, F) (n=4). Mean value of the control was considered 100% in each case. Statistical significance was determined by a 2‐tailed Student's t test. Data are mean±SEM. ns: P>0.05. *P≤0.05. HUVEC indicates human umbilical vascular endothelial cell; and mr‐NPNT, recombinant mouse nephronectin.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Am. Heart Assoc.