FIGURE

Fig. 5

ID

ZDB-FIG-250311-80

Publication

Patra et al., 2025 - Nephronectin Is Required for Vascularization in Zebrafish and Sufficient to Promote Mammalian Vessel-Like Structures in Hydrogels for Tissue Engineering

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Fig. 5

NPNT interacts with ITGAV and ITGB3. A and B, Representative brightfield images of whole‐mount in situ hybridized embryos reveal itgav (A) or itgb3.1 (B) expression. Arrows and arrowheads indicate itgav or itgb3.1 expression in the caudal vein plexus region and posterior somites, respectively. C, Western blot analysis of total cell lysate and immune‐complexes immunoprecipitated with anti‐Flag antibody from cultured HEK‐293 cells transfected with Flag‐tagged NPNT (lane‐1), ITGAV/ITGB3 (lane‐2), and both Flag‐tagged NPNT and ITGAV/ITGB3 (lane‐3) constructs. Cell lysate and immune complexes were blotted for ITGAV and ITGB3. CVP indicates caudal vein plexus; IP, immunoprecipitate; ITGAV, integrin alpha‐V; ITGB3, integrin beta‐3 NC, notochord; NPNT, nephronectin; and NT, neural tube.

Expression Data

Genes:	itgav itgb3b
Fish:	AB
Anatomical Terms:	caudal vein plexus somite
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Am. Heart Assoc.