FIGURE

Fig. 3

ID

ZDB-FIG-250311-78

Publication

Patra et al., 2025 - Nephronectin Is Required for Vascularization in Zebrafish and Sufficient to Promote Mammalian Vessel-Like Structures in Hydrogels for Tissue Engineering

Other Figures

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Fig. 3

Decreased endothelial cell proliferation in the caudal vein plexus of npnta morphants. A Maximum projections of confocal images of 32 hpf whole‐mount embryos stained for 5‐bromo‐2′‐deoxyuridine (red; marking proliferating cells) and EGFP (pseudo color; labeling ECs). Arrowheads indicate 5‐bromo‐2′‐deoxyuridine+/EGFP+ cells. B, Quantitative analysis of EC proliferation at the caudal vein plexus. Graph representing the ratio of EGFP+/ethynyl‐2′‐deoxyuridine+ ECs relative to the total number of EGFP+ ECs at the CVP. A total of 24 control and 21 npnta morphants from 2 independent experiments were analyzed. The mean EC proliferative index for control embryos was considered as 100%. Statistical significance was determined by a 2‐tailed Student's t test. Data are mean±SEM. ***P≤0.001. CVP indicates caudal vein plexus; EC, endothelial cell; EGFP, enhanced green fluorescent protein; hpf, hours post fertilization; and MO2 spliced blocking morpholino.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ci1Tg + MO2-npnta (AB)
Knockdown Reagent:	MO2-npnta
Observed In:	caudal vein plexus cell population proliferation
Stage:	Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Am. Heart Assoc.