Fig 7

HIF-1α inhibitor PX478 enhances the antiviral ability against SVCV infection and inhibits SVCV replication in zebrafish. (A) Representative images of zebrafish larvae (3 dpf) treated with DMSO (vehicle control) or PX478 (10 µM) for 12 h, followed by infected with SVCV (5 × 10⁷ TCID₅₀/mL) for 36 h. (B) Survival (Kaplan-Meier curve) of zebrafish larvae (3 dpf) (n = 30 per group) treated with PX478 (10 µM) for 12 h, followed by infected with or without SVCV (5 × 10⁷ TCID₅₀/mL) for 52 h. (C, D) qPCR analysis of phd3 (C) and vegfaa (D) mRNA in zebrafish larvae (3 dpf) treated with DMSO (vehicle control) or PX478 (10 µM) for 12 h, followed by infected with or without SVCV (5 × 10⁷ TCID₅₀/mL) for 24 h. (E-I) qPCR analysis of ifn1 (E), ifn2 (F), mxb (G), mxc (H), and lta (I) mRNA in zebrafish larvae (3 dpf) treated with DMSO (vehicle control) or PX478(10 µM) for 12 h, followed by infected with or without SVCV (5 × 10⁷ TCID₅₀/mL) for 24 h. (J) qPCR analysis of SVCV-G mRNA in zebrafish larvae (3 dpf) treated with DMSO (vehicle control) or PX478(10 µM) for 12 h, followed by infected with or without SVCV (5 × 10⁷ TCID₅₀/mL) for 24 h.