FIGURE

Fig. 2.

ID

ZDB-FIG-241218-83

Publication

Siddiqui et al., 2024 - Stat3 mediates Fyn kinase-driven dopaminergic neurodegeneration and microglia activation

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2.

Zebrafish neural FynY531F overexpression drives dopaminergic neuron loss and mitochondria accumulation. (A) Left: live confocal imaging of ventral diencephalon (vDC) neuron cluster eGFP dopaminergic neurons in 3 dpf and 5 dpf control dat:eGFP and elavl3:Gal4; UAS:fynY531F larvae. Top right: vDC in 5 dpf larval brain. Diagram adapted from Kalyn et al. (2019) and created with BioRender.com. Bottom right: quantification of vDC eGFP neuronal cell bodies in 5 dpf control dat:eGFP and dat:eGFP; elavl3:Gal4; UAS:fynY531F larvae (n=4). (B) Left: live confocal imaging of eGFP and mCherry in vDC neurons in 3 dpf control dat:mitoRFP; dat:eGFP; elavl3:Gal4 and dat:mitoRFP; dat:eGFP; elavl3:Gal4; UAS:fynY531F larval brain. Right: quantification of overlap of eGFP and mCherry signal in vDC neuron cell bodies in 3 dpf control dat:mitoRFP; dat:eGFP; elavl3:Gal4 and dat:mitoRFP; dat:eGFP; elavl3:Gal4; UAS:fynY531F larvae (n=6). (C) Left: live confocal imaging of eGFP and mCherry in vDC neurons in 5 dpf control dat:mitoRFP; dat:eGFP; elavl3:Gal4 and dat:mitoRFP; dat:eGFP; elavl3:Gal4; UAS:fynY531F larval brain. Right: quantification of overlap of eGFP and mCherry signal in vDC neuron cell bodies in 5 dpf control dat:mitoRFP; dat:eGFP; elavl3:Gal4 and dat:mitoRFP; dat:eGFP; elavl3:Gal4; UAS:fynY531F larvae (n=6). Statistical analysis was performed with two-tailed unpaired Student’s t-test. Bars represent mean±s.e.m. *P<0.05; **P<0.01; ***P<0.001. Scale bars: 50 µm (white); 100 µm (black).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.