Figure 3

Suppression of YAP signaling promotes LPC-mediated liver regeneration, similar to PPARα activation. (A, B) qRT-PCR data showing the relative expression levels of hepatocyte (cyp7a1, gc, serpina1, ces2) and BEC (her9, epcam) markers and YAP target genes (ccn1, ccn2) between DMSO- and K-975-treated Tg(fabp10a:pt-β-catenin) livers at 14 dpf. (C) Section in situ hybridization images showing the expression of hepatocyte (cyp7a1, gc) and BEC (epcam) markers in K-975-treated Tg(fabp10a:pt-β-catenin) livers at 14 dpf. For quantification, larvae were grouped into +/− based on gene expression levels. Numbers in the upper right corner indicate the proportion of larvae exhibiting the phenotype shown. (D) Immunofluorescence images showing Bhmt expression in 13-dpf Tg(fabp10a:pt-β-catenin) livers treated with K-975. Based on the level of Bhmt expression, larvae were divided into three groups: +++/++/+. Data are represented as mean ± SD (A, B). ns not significant; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; statistical significance was calculated using an unpaired two-tailed t-test (A, B), Fisher’s exact test (C), and chi-square test (D). Dashed lines outline the livers (C, D). Scale bars, 50 μm.