FIGURE

Fig. 12

ID

ZDB-FIG-230405-19

Publication

Tan et al., 2022 - Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear

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Fig. 12

Effects of sp5a and sp5l knockdown on early prosensory development. (A–H) Dorsal views (medial to the top, anterior to the left) of otic tissue showing expression of atoh1b at 12 and 14 hpf in wild-type control embryos, pax2a−/− mutants, sp5a−/−; sp5l−/− double mutants, and pax2a−/−; sp5a−/−; sp5l−/− triple mutants. Scale bar (A), 50 μm. (I–P) Dorsal views (anterior to left) showing expression of atoh1b at 12 and 14 hpf following injection of sp5a-MO and/or sp5l-MO at the one-cell stage, as indicated at the top.

Expression Data

Gene:	atoh1a
Fish:	pax2a^tu29a/tu29a AB + MO1-sp5a AB + MO2-sp5l sp5a^x69/x69 + MO2-sp5l sp5a^x69/x69; sp5l^x70/x70 sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l pax2a^tu29a/tu29a; sp5a^x69/x69; sp5l^x70/x70 (all 7)
Knockdown Reagents:	MO1-sp5a MO2-sp5l
Anatomical Term:	otic placode
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax2a^tu29a/tu29a AB + MO1-sp5a AB + MO2-sp5l sp5a^x69/x69 + MO2-sp5l sp5a^x69/x69; sp5l^x70/x70 sp5a^x69/x69; sp5l^x70/x70 + MO1-sp5a + MO2-sp5l pax2a^tu29a/tu29a; sp5a^x69/x69; sp5l^x70/x70 (all 7)
Knockdown Reagents:	MO1-sp5a MO2-sp5l
Observed In:	otic placode
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 492, Tan, A.L., Mohanty, S., Guo, J., Lekven, A.C., Riley, B.B., Pax2a, Sp5a and Sp5l act downstream of Fgf and Wnt to coordinate sensory-neural patterning in the inner ear, 139-153, Copyright (2022) with permission from Elsevier. Full text @ Dev. Biol.