FIGURE

Figure 1

ID

ZDB-FIG-210725-110

Publication

Jeong et al., 2021 - Optogenetic Manipulation of Olfactory Responses in Transgenic Zebrafish: A Neurobiological and Behavioral Study

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Figure 1

Generation of a transgenic zebrafish expressing ChR2-Kaede in olfactory sensory neurons. (A) Generation of transgenic fish using the expression construct of omp-driven ChR2-Kaede with transposase mRNA. (B) Schematic view of the transgenic zebrafish expressing channelrhodopsin-2 (ChR2) in olfactory sensory neurons. The green fluorescence of Kaede was photo-converted to red color, as an indicator for activation. (C,D) Ventral view of the head of the transgenic zebrafish embryos at 48 hpf and 72 hpf. (E,F) Dorsal view of the transgenic fish at 168 hpf. The Kaede protein of OSNs was photoconverted by UV light green to red fluorescence (F). Asterisks indicate the nasal pit, and arrows indicate the olfactory epithelium. m, mouth; OB, olfactory bulb. Scale bars, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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