FIGURE

Fig. 6

ID

ZDB-FIG-151215-14

Publication

Li et al., 2015 - Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development

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Fig. 6

The expression of Nr2f1b is upregulated by notch signaling. a, b nr2f1b expression is increased at 24 hpf embryos after treatment with DAPT as compared to DMSO control embryos by in situ hybridization. d, e nr2f1b expression is upregulated in rbpsuh morphants at 24 hpf embryos. c, f Quantification by qPCR showed the increased expression of nr2f1b in rbpsuh morphants or DAPT treated embryos at 24 hpf significantly. g-j Representative confocal images showing the number of nuclei per ISV at 30 hpf in g wild-type (wt) embryos, h nr2f1b ^MO, i rbpsuh ^MO, and j rbpsuh ^MO with nr2f1b ^MO. k Quantitation of the average number of cells per ISV in single and double morphants. ***refers to p < 0.0005 by an unpaired student′s t-test. Scale bars represent 100 µm in a, b, d, e and 50 µm in g-j.

Expression Data

Gene:	nr2f1b
Fish:	TL TL + MO4-rbpja,rbpjb
Condition:	chemical treatment: DAPT
Knockdown Reagent:	MO4-rbpja,rbpjb
Anatomical Terms:	blood vessel whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TL ci5Tg; y7Tg + MO4-nr2f1b TL + MO4-rbpja,rbpjb ci5Tg; y7Tg + MO4-rbpja,rbpjb ci5Tg; y7Tg + MO4-nr2f1b + MO4-rbpja,rbpjb
Condition:	chemical treatment: DAPT
Knockdown Reagents:	MO4-nr2f1b MO4-rbpja,rbpjb
Observed In:	blood vessel intersegmental vessel cell whole organism
Stage Range:	Prim-5 to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biomed. Sci.