PUBLICATION

Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development

Authors
Li, R.F., Wu, T.Y., Mou, Y.Z., Wang, Y.S., Chen, C.L., Wu, C.Y.
ID
ZDB-PUB-151119-11
Date
2015
Source
Journal of Biomedical Science   22: 104 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Veins/embryology*
  • Neovascularization, Physiologic/physiology*
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism
  • Signal Transduction/physiology
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Mice
  • Zebrafish/embryology*
  • Animals
(all 12)
PubMed
26572615 Full text @ J. Biomed. Sci.
Abstract
Background The specification of vein and the patterning of intersegmental vessels (ISV) controlled by transcription factor is not fully characterized. The orphan nuclear receptor Chicken ovalbumin upstream promoter transcription factor II (CoupTFII, a.k.a NR2F2) positively regulates vein identity in mice. In this study, we show that nr2f1b is important for vein and tip cell identity during zebrafish development.
Results Nr2f1b mRNA is expressed in ventral lateral mesoderm at 15S stage and in vessels at 24 hpf consistent with a role in early vascular specification. Morpholino knockdown of nr2f1b results in a decrease in both vein cell number and expression of the vein specific marker flt4 and mrc1, suggested its role in venous specification. We also show loss of nr2f1b reduced ISV cell number and impairs ISV growth, which is likely due to the impairment of angiogenic cells migration and/or proliferation by time-lapse imaging. Consequently, nr2f1b morphants showed pericardial edema and circulation defects. Overexpression of nr2f1b under the fli promoter increases the number of venous cells and ISV endothelial cells indicated the function of nr2f1b is required and necessary for vascular development. We further showed that nr2f1b likely interact with Notch signalling. nr2f1b expression is increased in rbpsuh morphants and DAPT-treatment embryos suggested nr2f1b is negatively regulated by Notch activity.
Conclusions We show nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development, which is mediated by Notch signalings.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
TLchemical treatment: DAPTPrim-5
TLchemical treatment: DAPTPrim-5
TL + MO4-nr2f1bcontrolPrim-5
TL + MO4-nr2f1bcontrolPrim-5
TL + MO4-nr2f1bcontrolPrim-5
TL + MO4-nr2f1bstandard conditionsProtruding-mouth
TL + MO4-nr2f1bstandard conditionsProtruding-mouth
TL + MO4-rbpja,rbpjbcontrolPrim-5
TL + MO4-rbpja,rbpjbcontrolPrim-5
ci5Tg; y7Tg + MO4-nr2f1bstandard conditionsPrim-15
1 - 10 of 39
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ci5TgTransgenic Insertion
    la116TgTransgenic Insertion
      sd2TgTransgenic Insertion
        y1TgTransgenic Insertion
          y7TgTransgenic Insertion
            1 - 5 of 5
            Show
            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            nr2f1bMO2-nr2f1bMRPHLNO
            nr2f1bMO3-nr2f1bMRPHLNO
            nr2f1bMO4-nr2f1bMRPHLNO
            rbpjaMO4-rbpja,rbpjbMRPHLNO
            rbpjbMO4-rbpja,rbpjbMRPHLNO
            1 - 5 of 5
            Show
            Fish
            Fish
            ci5Tg; y7Tg
            ci5Tg; y7Tg + MO4-nr2f1b
            ci5Tg; y7Tg + MO4-nr2f1b + MO4-rbpja,rbpjb
            ci5Tg; y7Tg + MO4-rbpja,rbpjb
            la116Tg
            la116Tg + MO2-nr2f1b
            la116Tg + MO3-nr2f1b
            la116Tg + MO4-nr2f1b
            sd2Tg; y1Tg
            sd2Tg; y1Tg + MO4-nr2f1b
            1 - 10 of 15
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            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            DsRedEFGDsRed
            EGFPEFGEGFP
            GFPEFGGFP
            mCherryEFGmCherry
            1 - 4 of 4
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            Mapping
            No data available
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            Citation
            Li, R.F., Wu, T.Y., Mou, Y.Z., Wang, Y.S., Chen, C.L., Wu, C.Y. (2015) Nr2f1b control venous specification and angiogenic patterning during zebrafish vascular development. Journal of Biomedical Science. 22:104.