FIGURE

Fig. 5

ID

ZDB-FIG-111201-58

Publication

Bachmann-Gagescu et al., 2011 - The Ciliopathy Gene cc2d2a controls Zebrafish Photoreceptor Outer Segment Development Through a Role in Rab8-dependent Vesicle Trafficking

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Fig. 5

Selective trafficking defects in cc2d2a-/- photoreceptors. (A and B) BOPS localization (BOPS antibody, red) in wild-type (A) and cc2d2a-/- (B) photoreceptors. (C and D) Rhodopsin localization (4D2 antibody, red) in wild-type (C) and cc2d2a-/- (D) photoreceptors. Outer segments are indicated by brackets. Mislocalized photopigment (opsin) is indicated by white arrows. (E and F) Transducin (red) localization by immunofluorescence is restricted to the outer segments (indicated by brackets) in both cc2d2a+/+ and cc2d2a-/- photoreceptors. (G and H) Ribeye (red) localization at the synapse is indistinguishable between cc2d2a+/+ and cc2d2a-/- photoreceptors. (I-I′′) BOPS staining [red in (I)] and Golgi expression of tg(gnat2:Man2a-RFP, green) do not significantly overlap [see arrowheads in (I) and (I′′) for BOPS and arrow in (I) and (I′) for Golgi]. (J) Rhodopsin (4D2 antibody, red, arrow) is mislocalized between the nucleus (DAPI, blue) and the subcortical actin network stained with phalloidin (green, arrowhead). All images are single-confocal sections of 5 d.p.f. cryosections. Scale bars are 4 µm in all panels.

Expression Data

Genes:	ctbp2a gnat2 opn1sw2 rho
Antibodies:	Ab1-opn1sw2 Ab5-rho
Fish:	WT cc2d2a^w38/w38 cc2d2a^w38/+
Anatomical Terms:	eye photoreceptor cell cytoskeleton of presynaptic active zone retinal cone cell cytoplasm retinal cone cell photoreceptor outer segment retinal rod cell cytoplasm retinal rod cell photoreceptor outer segment
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cc2d2a^w38/w38
Observed In:	photoreceptor cell outer segment organization
Stage:	Day 5

Phenotype Detail

Acknowledgments

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