(A) Platelet distribution of zebrafish in each group, with tail platelet aggregation in the red frame. (B) The number of platelets circulating in the caudal. (C) The number of platelets in the heart. ^#p < 0.05, compared with the blank control group; ** p < 0.01, compared with the arachidonic acid (AA) group.

Representative images of heart region (A) and trunk region (B) of zebrafish stained with O-dianisidine in each group. The black frame indicates erythrocyte aggregation; the lower right corner was labeled as the number of zebrafish with erythrocyte staining in the trunk region/total number of zebrafish. Erythrocyte staining area (C) and staining intensity (D) in the heart. ^###p < 0.001, compared with the blank control group; * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with the AA group.

(A) Representative images of the heart region, (B) cardiac erythrocyte fluorescence area, (C) cardiac erythrocyte fluorescence intensity, and (D) heart rate. ^##p < 0.01 and ^###p < 0.001, compared with the blank control group; * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with the AA group.

(A) Hemodynamic images of the caudal arteries of zebrafish in each group. (B) Histogram of blood flow velocity in the caudal artery. ^###p < 0.001, compared with the blank control group; *** p < 0.001, compared with the AA group.

The levels of (A) TXA₂, (B) vWF, and (C) D-dimer in zebrafish. ^###p < 0.001, compared with the blank control group; *** p < 0.001, compared with the AA group.

(A) Principal Component Analysis (PCA) plot, (B) Venn diagram of common and specific differentially expressed genes (DEGs) between different groups, (C) statistical histogram of DEGs, and (D) intersection DEG trend analysis. Orange boxes indicate the DEGs with significant change trends.

The effect of Brevianamide F on intersection DEGs with significant trend changes. (A) The GO function enrichment analysis. The vertical axis was a GO term which included three categories: biological process (BP), cellular component (CC), and molecular function (MF). The horizontal axis was the -log₁₀(p-value) of the GO items. (B) The KEGG pathway enrichment analysis. The vertical axis was the name of the signaling pathway and the horizontal axis was the enrichment score. The size of the scatter represented the number of DEGs of the item, and the redder the color, the smaller the enrichment p-value and the greater the significance of the item.

Effects of Brevianamide F on the expression of genes related to the MAPK pathway and coagulation cascade; ^#p < 0.05, ^##p < 0.01, and ^###p < 0.001, compared with the blank control group; * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with the AA group.

The molecular docking results of Brevianamide F with key targets. Each result contains an overall picture and a local zoomed-in picture. Red color indicates the molecular structure of Brevianamide F. Hydrogen bonds are shown in yellow and amino acid residues are shown in blue.