FIGURE SUMMARY
Title

Diverse Engraftment Capability of Neuroblastoma Cell Lines in Zebrafish Larvae

Authors
Lawrence, J.M., Tan, S.H., Kim, D.C., Tan, K.E., Schroeder, S.E., Yeo, K.S., Schaefer, M.A., Sosic, A.M., Zhu, S.
Source
Full text @ Zebrafish
Fig. 1

Kelly cells successfully engraft in the HBV and PCS of 48 hpf zebrafish, with an initial location preference appreciated for the HBV. (A) Representative HBV xenografted fish imaged at 1, 3, and 6 DPI. (B) Quantification of tumor volume change depicted in (A); whiskers extending to 10th and 90th percentile. (C) Representative PCS xenografted fish imaged at 1, 3, and 6 DPI. (D) Quantification of tumor volume change depicted in (C); whiskers extending to 10th and 90th percentile. (E) Comparison of tumor growth between the HBV and PCS at 1, 3, and 6 DPI. Tumor volume calculated at each timepoint by multiplication of fluorescent area and mean intensity value at imaging. Magnitude of change at 3 and 6 DPI normalized to tumor volume at 1 DPI for individual fish. *p < 0.05, **p < 0.02, ***p < 0.01, ns not significant.

Fig. 2

Be(2)-C cells fail to engraft in the HBV and PCS of 48 hpf zebrafish, with a location preference appreciated for the HBV. (A) Representative HBV xenografted fish imaged at 1, 3, and 6 DPI. (B) Quantification of tumor volume change depicted in (A); whiskers extending to 10th and 90th percentile. (C) Representative PCS xenografted fish imaged at 1, 3, and 6 DPI. (D) Quantification of tumor volume change depicted in (C); whiskers extending to 10th and 90th percentile. (E) Comparison of tumor growth between the HBV and PCS at 1, 3, and 6 DPI. Tumor volume calculated at each timepoint by multiplication of fluorescent area and mean intensity value at imaging. Magnitude of change at 3 and 6 DPI normalized to tumor volume at 1 DPI for individual fish. *p < 0.05, **p < 0.02, ***p < 0.01.

Fig. 3

SY-5Y cells fail to engraft in the HBV and PCS of 48 hpf zebrafish, with near complete loss of cells in the PCS. (A) Representative HBV xenografted fish imaged at 1, 3, and 6 DPI. (B) Quantification of tumor volume change depicted in (A); whiskers extending to 10th and 90th percentile. (C) Representative PCS xenografted fish imaged at 1, 3, and 6 DPI. (D) Quantification of tumor volume change depicted in (C); whiskers extending to 10th and 90th percentile. (E) Comparison of tumor growth between the HBV and PCS at 1, 3, and 6 DPI. Tumor volume calculated at each timepoint by multiplication of fluorescent area and mean intensity value at imaging. Magnitude of change at 3 and 6 DPI normalized to tumor volume at 1 DPI for individual fish. *p < 0.05, **p < 0.02, ***p < 0.01.

Fig. 4

SK-N-AS cells successfully engraft in the HBV and PCS of 48 hpf zebrafish, with an initial location preference appreciated for the HBV. (A) Representative HBV xenografted fish imaged at 1, 3, and 6 DPI. (B) Quantification of tumor volume change depicted in (A); whiskers extending to 10th and 90th percentile. (C) Representative PCS xenografted fish imaged at 1, 3, and 6 DPI. (D) Quantification of tumor volume change depicted in (C); whiskers extending to 10th and 90th percentile. (E) Comparison of tumor growth between the HBV and PCS at 1, 3, and 6 DPI. Tumor volume calculated at each timepoint by multiplication of fluorescent area and mean intensity value at imaging. Magnitude of change at 3 and 6 DPI normalized to tumor volume at 1 DPI for individual fish. *p < 0.05, **p < 0.02, ***p < 0.01, ns, not significant.

Fig. 5

Kelly and SK-N-AS neuroblastoma cells engraft in the HBV and PCS of 48 hpf zebrafish while Be(2)-C tumor volume remains static and SY-5Y fails to engraft. (A, B) Comparison of tumor magnitude of change for four cell lines in the HBV at 3 DPI (A) and 6 DPI (B). (C, D) Comparison of tumor magnitude of change in the PCS for four cell lines at 3 DPI (C) and 6 DPI (D). Median magnitude of change at 3 and 6 DPI normalized to tumor volume at 1 DPI. ***p < 0.01, ns not significant.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Zebrafish
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Please check the highlighted fields and try again.
Thank you for submitting comments. Your input has been emailed to ZFIN curators who may contact you if additional information is required.
Oops. Something went wrong. Please try again later.