Fig. 1

Images depicting the tailfin transection method. a-b) demonstrate tailfin transection with fluorescence; c-d) depict tailfin transection with brightfield microscopy to be see the areas transected.

Fig. 2

Amino acid sequence alignment of human vs zebrafish cytokines. Values are expressed as percentage of total amino acids assessed.

Fig. 3

Representative immunofluorescence images depicting the Tumor necrosis factor-α (TNF-α) response to tailfin transection in zebrafish larvae in a) controls, b) 1hr, c) 2hr, d) 4hr, and e) 24hr post injury. Quantified data and statistical results are presented in frame f (colour correlates to protein depiction in Fig. 9). Graph represents integrated density fluorescent intensity measurements of the defined area of the tailfin. Data are presented as mean ± SEM. Scale bar represents 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 4

Representative immunofluorescence images depicting the interleukin-1β (IL-1β) response to tailfin transection in zebrafish larvae in a) controls, b) 1hr, c) 2hr, d) 4hr, and e) 24hr post injury. Quantified data and statistical results are presented in frame f (colour correlates to protein depiction in Fig. 9). Graph represents integrated density fluorescent intensity measurements of the defined area of the tailfin. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01. Scale bar represents 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 5

Representative immunofluorescence images depicting the interleukin-6 (IL-6) response to tailfin transection in zebrafish larvae in a) controls, b) 1hr, c) 2hr, d) 4hr, and e) 24hr post injury. Quantified data and statistical results are presented in frame f (colour correlates to protein depiction in Fig. 9). Graph represents integrated density fluorescent intensity measurements of the defined area of the tailfin. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Scale bar represents 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 6

Representative immunofluorescence images depicting the monocyte chemoattractant protein-1 (MCP-1) response to tailfin transection in zebrafish larvae in a) controls, b) 1hr, c) 2hr, d) 4hr, and e) 24hr post injury. Quantified data and statistical results are presented in frame f (colour correlates to protein depiction in Fig. 9). Graph represents integrated density fluorescent intensity measurements of the defined area of the tailfin. Data are presented as mean ± SEM. Scale bar represents 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 7

Representative immunofluorescence images depicting the interleukin-10 (IL-10) response to tailfin transection in zebrafish larvae in a) controls, b) 1hr, c) 2hr, d) 4hr, and e) 24hr post injury. Quantified data and statistical results are presented in frame f (colour correlates to protein depiction in Fig. 9). Graph represents integrated density fluorescent intensity measurements of the defined area of the tailfin. Data are presented as mean ± SEM. Scale bar represents 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 8

Representative immunofluorescence images depicting the macrophage migration inhibitory factor (MIF) response to tailfin transection in zebrafish larvae in a) controls, b) 1hr, c) 2hr, d) 4hr, and e) 24hr post injury. Quantified data and statistical results are presented in frame f (colour correlates to protein depiction in Fig. 9). Graph represents integrated density fluorescent intensity measurements of the defined area of the tailfin. Data are presented as mean ± SEM. *p < 0.05; **p < 0.01. Scale bar represents 100 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 9

Representative graphs comparing the (a) zebrafish immunofluorescent staining results with (b) general and predicted trends observed for cytokine responses to acute injury in human and rodent models as described in the text below.

Acknowledgments
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