PUBLICATION

Control of muscle cell-type specification in the zebrafish embryo by hedgehog signalling

Authors

Lewis, K.E., Currie, P.D., Roy, S., Schauerte, H., Haffter, P., and Ingham, P.W.

ID

ZDB-PUB-991216-1

Date

1999

Source

Developmental Biology 216(2): 469-480 (Journal)

Registered Authors

Currie, Peter D., Haffter, Pascal, Ingham, Philip, Lewis, Katharine E., Roy, Sudipto, Schauerte, Heike

Keywords

Zebrafish; Patched1; Sonic hedgehog; Gli2; U-type mutants; Muscle fibre type; MyoD; Myosin heavy chain; You; You-too; Sonic you; Chameleon; U-boot; gene control; muscle cell; cell type; cell specificity; signal transduction; sonic hedgehog protein

MeSH Terms

Mutation
RNA, Messenger/metabolism
Transcription Factors/metabolism
Membrane Proteins/genetics
Trans-Activators*
Myosins/metabolism
In Situ Hybridization
Fluorescent Antibody Technique
Zebrafish
MyoD Protein/genetics
Animals
Cell Differentiation
Receptors, Cell Surface
Muscles/embryology*
Muscles/metabolism
Gene Expression Regulation, Developmental
Kruppel-Like Transcription Factors
Somites/metabolism
Hedgehog Proteins
Proteins/metabolism*
Signal Transduction*

(all 21)

PubMed

10642786 Full text @ Dev. Biol.

Abstract

The specification of different muscle cell types in the zebrafish embryo requires signals that emanate from the axial mesoderm. In previous studies we and others have shown that overexpression of different members of the Hedgehog protein family can induce the differentiation of two types of slow-twitch muscles, the superficially located slow-twitch fibres and the medially located muscle pioneer cells. Here we have investigated the requirement for Hedgehog signalling in the specification of these distinct muscle cell types in two ways: first, by characterising the effects on target gene expression and muscle cell differentiation of the u-type mutants, members of a phenotypic group previously implicated in Hedgehog signalling, and second, by analysing the effects of overexpression of the Patched1 protein, a negative regulator of Hedgehog signalling. Our results support the idea that most u-type genes are required for Hedgehog signalling and indicate that while such signalling is essential for slow myocyte differentiation, the loss of activity of one signal, Sonic hedgehog, can be partially compensated for by other Hedgehog family proteins.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
myod1	Df(Chr07)t4/t4	standard conditions	10-13 somites to 14-19 somites		adaxial cell	ISH	Fig. 3
myod1	Df(Chr07)t4/t4	standard conditions	5-9 somites		adaxial cell	ISH	Fig. 2
myod1	Df(Chr07)t4/t4	standard conditions	5-9 somites		somite	ISH	Fig. 2
myod1	Df(Chr07)t4/t4	standard conditions	10-13 somites to 14-19 somites		tail bud	ISH	Fig. 3
myod1	disp1^tm15a/tm15a	standard conditions	10-13 somites to 14-19 somites		adaxial cell	ISH	Fig. 3
myod1	disp1^tm15a/tm15a	standard conditions	5-9 somites		adaxial cell	ISH	Fig. 2
myod1	disp1^tm15a/tm15a	standard conditions	5-9 somites		somite	ISH	Fig. 2
myod1	disp1^tm15a/tm15a	standard conditions	10-13 somites to 14-19 somites		tail bud	ISH	Fig. 3
myod1	gli2a^ty119/ty119	standard conditions	75%-epiboly to Bud		adaxial cell	ISH	Fig. 1
myod1	gli2a^ty119/ty119	standard conditions	10-13 somites to 14-19 somites	Not Detected	adaxial cell	ISH	Fig. 3

1 - 10 of 55

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Phenotype

Mutations / Transgenics

Allele	Type	Affected Genomic Region
t4	Deficiency	shha
tm15a	Point Mutation	disp1
tp39	Point Mutation	prdm1a
ty97	Point Mutation	scube2
ty119	Point Mutation	gli2a

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
Df(Chr07)t4/t4
disp1^tm15a/tm15a
gli2a^ty119/ty119
prdm1a^tp39/tp39
scube2^ty97/ty97
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-eng	monoclonal	en1a en1b en2a en2b	IgG1	Mouse
Ab1-smyhc	monoclonal		IgG2b	Mouse
Ab-EB165	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Mapping

Lewis et al., 1999 ZDB-PUB-991216-1 PMID:10642786

Control of muscle cell-type specification in the zebrafish embryo by hedgehog signalling

Lewis et al., 1999

ZDB-PUB-991216-1
PMID:10642786