PUBLICATION

Mutations affecting the cardiovascular system and other internal organs in zebrafish

Authors
Chen, J.N., Haffter, P., Odenthal, J., Vogelsang, E., Brand, M., van Eeden, F.J., Furutani-Seiki, M., Granato, M., Hammerschmidt, M., Heisenberg, C.P., Jiang, Y.J., Kane, D.A., Kelsh, R.N., Mullins, M.C., and Nüsslein-Volhard, C.
ID
ZDB-PUB-970210-25
Date
1996
Source
Development (Cambridge, England)   123: 293-302 (Journal)
Registered Authors
Brand, Michael, Chen, Jau-Nian, Furutani-Seiki, Makoto, Granato, Michael, Haffter, Pascal, Hammerschmidt, Matthias, Heisenberg, Carl-Philipp, Jiang, Yun-Jin, Kane, Donald A., Kelsh, Robert, Mullins, Mary C., Nüsslein-Volhard, Christiane, Odenthal, Joerg, van Eeden, Freek
Keywords
heart; circulation; cardiovascular system; intestine; liver; kidney; zebrafish
MeSH Terms
  • Heart Rate/genetics
  • Mutation*
  • Cardiovascular System/embryology*
  • Myocardial Contraction/genetics
  • Animals
  • Body Patterning/genetics
  • Heart Atria/abnormalities
  • Heart Atria/embryology
  • Cardiomegaly/embryology
  • Cardiomegaly/genetics
  • Heart Ventricles/abnormalities
  • Heart Ventricles/embryology
  • Kidney/abnormalities
  • Kidney/embryology
  • Phenotype
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Heart Defects, Congenital/embryology
  • Heart Defects, Congenital/genetics
  • Intestines/abnormalities
  • Intestines/embryology
  • Liver/abnormalities
  • Liver/embryology
  • Embryonic Development
(all 24)
PubMed
9007249 Full text @ Development
Abstract
In a screen for early developmental mutants of the zebrafish, we have identified mutations specifically affecting the internal organs. We identified 53 mutations affecting the cardiovascular system. Nine of them affect specific landmarks of heart morphogenesis. Mutations in four genes cause a failure in the fusion of the bilateral heart primordia, resulting in cardia bifida. In lonely atrium, no heart venticle is visible and the atrium is directly fused to the outflow tract. In the overlooped mutant, the relative position of the two heart chambers is distorted. The heart is enormously enlarged in the santa mutant. In two mutants, scotch tape and superglue, the cardiac jelly between the two layers of the heart is significantly reduced. We also identified a number of mutations affecting the function of the heart. The mutations affecting heart function can be subdivided into two groups, one affecting heart contraction and another affecting the rhythm of the heart beat. Among the contractility group of mutants are 5 with no heart beat at all and 15 with a reduced heart beat of one or both chambers. 6 mutations are in the rhythmicity group and specifically affect the beating pattern of the heart. Mutations in two genes, bypass and kurzschluss, cause specific defects in the circulatory system. In addition to the heart mutants, we identified 23 mutations affecting the integrity of the liver, the intestine or the kidney. In this report, we demonstrate that it is feasible to screen for genes specific for the patterning or function of certain internal organs in the zebrafish. The mutations presented here could serve as an entry point to the establishment of a genetic hierarchy underlying organogenesis.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b109
    Small Deletion
    b212
      Unknown
      m58
        Small Deletion
        m93
          Point Mutation
          m116
            Unknown
            m118
              Unknown
              m139
                Point Mutation
                m158
                  Unknown
                  m229
                    Unknown
                    m276
                      Unknown
                      1 - 10 of 97
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab-S46monoclonalIgG1Mouse
                      1 - 1 of 1
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                      Orthology
                      No data available
                      Engineered Foreign Genes
                      No data available
                      Mapping
                      No data available