PUBLICATION

foxg1a is required for hair cell development and regeneration in the zebrafish lateral line

Authors
Bell, J.M., Turner, E.M., Biesemeyer, C., Vanderbeck, M.M., Hendricks, R., McGraw, H.F.
ID
ZDB-PUB-240921-3
Date
2024
Source
Biology Open   13(9): (Journal)
Registered Authors
Keywords
foxg1a, Development, Hair cells, Proliferation, Zebrafish
MeSH Terms
  • Zebrafish*
  • Cell Differentiation/genetics
  • Mutation
  • Regeneration*
  • Animals
  • Lateral Line System*
  • Gene Expression Regulation, Developmental
  • Cell Proliferation
  • Hair Cells, Auditory*/cytology
  • Hair Cells, Auditory*/metabolism
  • Hair Cells, Auditory*/physiology
  • Forkhead Transcription Factors*/genetics
  • Forkhead Transcription Factors*/metabolism
  • Zebrafish Proteins*/genetics
  • Zebrafish Proteins*/metabolism
(all 15)
PubMed
39301848 Full text @ Biol. Open
Abstract
Mechanosensory hair cells located in the inner ear mediate the sensations of hearing and balance. If damaged, mammalian inner ear hair cells are unable to regenerate, resulting in permanent sensory deficits. Aquatic vertebrates like zebrafish (Danio rerio) have a specialized class of mechanosensory hair cells found in the lateral line system, allowing them to sense changes in water current. Unlike mammalian inner ear hair cells, lateral line hair cells can robustly regenerate following damage. In mammals, the transcription factor Foxg1 functions to promote normal development of the inner ear. Foxg1a is expressed in lateral line sensory organs in zebrafish larvae, but its function during lateral line development and regeneration has not been investigated. Our study demonstrates that mutation of foxg1a results in slower posterior lateral line primordium migration and delayed neuromast formation. In developing and regenerating neuromasts, we find that loss of Foxg1a function results in reduced hair cell numbers, as well as decreased proliferation of neuromast cells. Foxg1a specifically regulates the development and regeneration of Islet1-labeled hair cells. These data suggest that Foxg1 may be a valuable target for investigation of clinical hair cell regeneration.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a266
    Complex
    ba2TgTransgenic Insertion
      nl1TgTransgenic Insertion
        sk85TgTransgenic Insertion
          w186TgTransgenic Insertion
            w215TgTransgenic Insertion
            w217TgTransgenic Insertion
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            No data available
            Antibodies
            Name Type Antigen Genes Isotypes Host Organism
            Ab1-islmonoclonalIgG1Mouse
            Ab1-otofmonoclonalIgG2aMouse
            Ab18-sox2polyclonal
              IgGRabbit
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              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              EGFPEFGEGFP
              EosEFGEos
              mCherryEFGmCherry
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              Mapping
              No data available