PUBLICATION

Zbtb16 mediates a switch between Fgf signalling regimes in the developing hindbrain

Authors
Leino, S.A., Constable, S.C.J., Streit, A., Wilkinson, D.G.
ID
ZDB-PUB-230830-44
Date
2023
Source
Development (Cambridge, England)   150(18): (Journal)
Registered Authors
Wilkinson, David
Keywords
Fgf signalling, Hindbrain, Neurogenesis, Zbtb16
MeSH Terms
  • Rhombencephalon
  • Transcription Factors/genetics
  • Zebrafish*/genetics
  • Neurogenesis*/genetics
  • Animals
  • Signal Transduction/genetics
(all 6)
PubMed
37642135 Full text @ Development
Abstract
Developing tissues are sequentially patterned by extracellular signals that are turned on and off at specific times. In the zebrafish hindbrain, fibroblast growth factor (Fgf) signalling has different roles at different developmental stages: in the early hindbrain, transient Fgf3 and Fgf8 signalling from rhombomere 4 is required for correct segmentation, whereas later, neuronal Fgf20 expression confines neurogenesis to specific spatial domains within each rhombomere. How the switch between these two signalling regimes is coordinated is not known. We present evidence that the Zbtb16 transcription factor is required for this transition to happen in an orderly fashion. Zbtb16 expression is high in the early anterior hindbrain, then gradually upregulated posteriorly and confined to neural progenitors. In mutants lacking functional Zbtb16, fgf3 expression fails to be downregulated and persists until a late stage, resulting in excess and more widespread Fgf signalling during neurogenesis. Accordingly, the spatial pattern of neurogenesis is disrupted in zbtb16 mutants. Our results reveal how the distinct stage-specific roles of Fgf signalling are coordinated in the zebrafish hindbrain.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fci3TgTransgenic Insertion
    fci10
      		Small Deletion
      fci11
        		Small Deletion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        fgf3MO1-fgf3MRPHLNO
        tp53MO4-tp53MRPHLNO
        zbtb16aTALEN1-zbtb16aTALEN
        zbtb16bMO1-zbtb16bMRPHLNO
        zbtb16bTALEN1-zbtb16bTALEN
        1 - 5 of 5
        Show
        Fish
        No data available
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        CitrineEFGCitrine
        1 - 1 of 1
        Show
        Mapping
        No data available
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        Citation
        Leino, S.A., Constable, S.C.J., Streit, A., Wilkinson, D.G. (2023) Zbtb16 mediates a switch between Fgf signalling regimes in the developing hindbrain. Development (Cambridge, England). 150(18):.