PUBLICATION

Endothelial versus pronephron fate decision is modulated by the transcription factors Cloche/Npas4l, Tal1, and Lmo2

Authors
Mattonet, K., Riemslagh, F.W., Guenther, S., Prummel, K.D., Kesavan, G., Hans, S., Ebersberger, I., Brand, M., Burger, A., Reischauer, S., Mosimann, C., Stainier, D.Y.R.
ID
ZDB-PUB-220901-7
Date
2022
Source
Science advances   8: eabn2082 (Journal)
Registered Authors
Brand, Michael, Burger, Alexa, Hans, Stefan, Kesavan, Gokul, Mosimann, Christian, Prummel, Karin, Reischauer, Sven, Stainier, Didier
Keywords
none
MeSH Terms
none
PubMed
36044573 Full text @ Sci Adv
Abstract
Endothelial specification is a key event during embryogenesis; however, when, and how, endothelial cells separate from other lineages is poorly understood. In zebrafish, Npas4l is indispensable for endothelial specification by inducing the expression of the transcription factor genes etsrp, tal1, and lmo2. We generated a knock-in reporter in zebrafish npas4l to visualize endothelial progenitors and their derivatives in wild-type and mutant embryos. Unexpectedly, we find that in npas4l mutants, npas4l reporter-expressing cells contribute to the pronephron tubules. Single-cell transcriptomics and live imaging of the early lateral plate mesoderm in wild-type embryos indeed reveals coexpression of endothelial and pronephron markers, a finding confirmed by creERT2-based lineage tracing. Increased contribution of npas4l reporter-expressing cells to pronephron tubules is also observed in tal1 and lmo2 mutants and is reversed in npas4l mutants injected with tal1 mRNA. Together, these data reveal that Npas4l/Tal1/Lmo2 regulate the fate decision between the endothelial and pronephron lineages.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
lmo2bns499/bns499; npas4lbns313Tg; c264Tg; y1Tgstandard conditionsPrim-5
lmo2bns499/bns499; npas4lbns313Tg; c264Tg; y1Tgstandard conditionsPrim-5
lmo2bns499/bns499; npas4lbns313Tg; c264Tg; y1Tgstandard conditionsPrim-5
npas4lbns313Tg; etsrpbns426/bns426; c264Tg; y1Tgstandard conditionsPrim-5
npas4lbns313Tg; etsrpbns426/bns426; c264Tg; y1Tgstandard conditionsPrim-5
npas4lbns313Tg; etsrpbns426/bns426; c264Tg; y1Tgstandard conditionsPrim-5
npas4lbns313Tg; etsrpbns426/bns426; c264Tg; y1Tgstandard conditionsPrim-5
npas4lbns423Tg/+; c264Tg; y1Tgstandard conditionsPrim-5
npas4lbns423Tg/+; c264Tg; y1Tgstandard conditionsPrim-5
npas4lbns423Tg/+; c264Tg; y1Tgstandard conditionsPrim-5
1 - 10 of 25
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
bns297
    Small Deletion
    bns313TgTransgenic Insertion
    bns422
      Small Deletion
      bns423TgTransgenic Insertion
      bns426
        Complex
        bns497
          Complex
          bns498
            Small Deletion
            bns499
              Indel
              bns500
                Small Deletion
                c264TgTransgenic Insertion
                  1 - 10 of 22
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  1 - 7 of 7
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                  Fish
                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  Ab1-atp1a1monoclonal
                    IgG2aMouse
                    Ab2-pax2apolyclonal
                      IgGRabbit
                      1 - 2 of 2
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                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CreEFGCre
                      DsRedEFGDsRed
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      NTREFGNTR
                      VenusEFGVenus
                      1 - 8 of 8
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                      Mapping
                      No data available