PUBLICATION

Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes

Authors

Eom, D.S., Patterson, L.B., Bostic, R.R., Parichy, D.M.

ID

ZDB-PUB-210504-1

Date

2021

Source

Developmental Biology 476: 314-327 (Journal)

Registered Authors

Eom, Dae Seok, Parichy, David M.

Keywords

Cell adhesion, Iridophore, JAM3, Melanophore, Pigment pattern, Zebrafish

Datasets

GEO:GSE131136

MeSH Terms

Animals
Zebrafish Proteins/genetics
Junctional Adhesion Molecule C/genetics*
Junctional Adhesion Molecule C/metabolism*
Morphogenesis
Zebrafish/genetics
Gene Expression Regulation, Developmental/genetics
Phenotype
Signal Transduction/genetics
Body Patterning/genetics*
Carrier Proteins/metabolism
Metamorphosis, Biological/genetics
Mutation/genetics
Pigmentation/genetics
Cell Adhesion Molecules/genetics
Cell Adhesion Molecules/metabolism
Immunoglobulins/genetics
Immunoglobulins/metabolism
Melanophores/metabolism
Neural Crest/cytology
Gene Expression/genetics

(all 21)

PubMed

33933422 Full text @ Dev. Biol.

Abstract

Adhesive interactions are essential for tissue patterning and morphogenesis yet difficult to study owing to functional redundancies across genes and gene families. A useful system in which to dissect roles for cell adhesion and adhesion-dependent signaling is the pattern formed by pigment cells in skin of adult zebrafish, in which stripes represent the arrangement of neural crest derived melanophores, cells homologous to melanocytes. In a forward genetic screen for adult pattern defects, we isolated the pissarro (psr) mutant, having a variegated phenotype of spots, as well as defects in adult fin and lens. We show that psr corresponds to junctional adhesion protein 3b (jam3b) encoding a zebrafish orthologue of the two immunoglobulin-like domain receptor JAM3 (JAM-C), known for roles in adhesion and signaling in other developing tissues, and for promoting metastatic behavior of human and murine melanoma cells. We found that zebrafish jam3b is expressed post-embryonically in a variety of cells including melanophores, and that jam3b mutants have defects in melanophore survival. Jam3b supported aggregation of cells in vitro and was required autonomously by melanophores for an adherent phenotype in vivo. Genetic analyses further indicated both overlapping and non-overlapping functions with the related receptor, Immunoglobulin superfamily 11 (Igsf11) and Kit receptor tyrosine kinase. These findings suggest a model for Jam3b function in zebrafish melanophores and hint at the complexity of adhesive interactions underlying pattern formation.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
actb2	WT	standard conditions	Adult	fin	RTPCR	Fig. 3
actb2	WT	standard conditions	Adult	hypodermis iridophore	RTPCR	Fig. 3
actb2	WT	standard conditions	Adult	hypodermis melanocyte	RTPCR	Fig. 3
actb2	WT	standard conditions	Adult	hypodermis xanthophore	RTPCR	Fig. 3
csf1ra	WT	standard conditions	Adult	fin	RTPCR	Fig. 3
csf1ra	WT	standard conditions	Adult	hypodermis xanthophore	RTPCR	Fig. 3
GFP	la116Tg; vp36rTg	standard conditions	Days 14-20	blood vessel endothelial cell	IFL	Fig. 3
igsf11	WT	standard conditions	Adult	fin	RTPCR	Fig. 3
igsf11	WT	standard conditions	Adult	hypodermis iridophore	RTPCR	Fig. 3
igsf11	WT	standard conditions	Adult	hypodermis melanocyte	RTPCR	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
gja5b^t1/+; jam3b^vp8re2/+	standard conditions	Adult	melanophore stripe spatial pattern, normal	Fig. 6
igsf11^wp15e1/+; jam3b^vp8re2/+	standard conditions	Adult	melanophore stripe irregular spatial pattern, abnormal	Fig. 6
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 7-13	hypodermis melanocyte irregularly shaped, abnormal	Fig. 1
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 7-13 to Days 30-44	melanocyte cell death increased occurrence, abnormal	Fig. 1
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 14-20	hypodermis has fewer parts of type melanocyte, abnormal	Fig. 1
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 14-20	hypodermis melanocyte irregularly shaped, abnormal	Fig. 1
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 21-29	hypodermis has fewer parts of type melanocyte, abnormal	Fig. 1
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 21-29	hypodermis melanocyte irregularly shaped, abnormal	Fig. 1
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 30-44	hypodermis has fewer parts of type melanocyte, abnormal	Fig. 1
jam3b^{utr8e1/utr8e1} (AB)	standard conditions	Days 30-44	hypodermis melanocyte irregularly shaped, abnormal	Fig. 1

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b5		Small Deletion	kita
j9s1		Point Mutation	ltk
la116Tg	Tg(kdrl:GFP)	Transgenic Insertion
t1		Point Mutation	gja5b
utr8e1		Unknown	jam3b
vc7		Point Mutation	mitfa
vp8re2		Unknown	jam3b
vp8re3		Unknown	jam3b
vp15rc4		Insertion	igsf11
vp15rc5		Small Deletion	igsf11

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
igsf11	CRISPR2-igsf11	CRISPR
jam3b	CRISPR1-jam3b	CRISPR

Fish

Fish
gja5b^t1/+; jam3b^vp8re2/+
igsf11^wp15e1/+; jam3b^vp8re2/+
jam3b^{utr8e1/utr8e1} (AB)
jam3b^{vp8re2/vp8re2} (WIK)
jam3b^{vp8re3/vp8re3} (WIK)
la116Tg; vp36rTg
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-igsf11	polyclonal	igsf11		Mouse

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GFP	EFG	GFP
mCherry	EFG	mCherry

Mapping

Eom et al., 2021 ZDB-PUB-210504-1 PMID:33933422

Immunoglobulin superfamily receptor junctional adhesion molecule 3 (Jam3) requirement for melanophore survival and patterning during formation of zebrafish stripes

Eom et al., 2021

ZDB-PUB-210504-1
PMID:33933422