PUBLICATION

Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves

Authors
Takamiya, M., Stegmaier, J., Kobitski, A.Y., Schott, B., Weger, B.D., Margariti, D., Cereceda Delgado, A.R., Gourain, V., Scherr, T., Yang, L., Sorge, S., Otte, J.C., Hartmann, V., van Wezel, J., Stotzka, R., Reinhard, T., Schlunck, G., Dickmeis, T., Rastegar, S., Mikut, R., Nienhaus, G.U., Strähle, U.
ID
ZDB-PUB-200620-15
Date
2020
Source
PLoS Genetics   16: e1008774 (Journal)
Registered Authors
Dickmeis, Thomas, Gourain, Victor, Mikut, Ralf, Otte, Jens, Rastegar, Sepand, Strähle, Uwe, Takamiya, Masanari, Weger, Benjamin
Keywords
none
MeSH Terms
  • Anterior Eye Segment/cytology
  • Anterior Eye Segment/embryology
  • Anterior Eye Segment/metabolism*
  • Neurons/cytology
  • Neurons/metabolism
  • Cell Movement
  • Neurogenesis*
  • Transforming Growth Factor beta/metabolism
  • Signal Transduction
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Mutation
  • PAX6 Transcription Factor/genetics
  • PAX6 Transcription Factor/metabolism*
  • Neural Crest/cytology
  • Neural Crest/embryology
  • Neural Crest/metabolism*
  • Zebrafish
  • Animals
(all 19)
PubMed
32555736 Full text @ PLoS Genet.
Abstract
Cranial neural crest (NC) contributes to the developing vertebrate eye. By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of migration. The first NC population migrates to the proximal and the second NC cell group populates the distal (anterior) part of the eye. By analysing zebrafish pax6a/b compound mutants presenting anterior segment dysgenesis, we demonstrate that Pax6a/b guide the two NC populations to distinct proximodistal locations. We further provide evidence that the lens whose formation is pax6a/b-dependent and lens-derived TGFβ signals contribute to the building of the anterior segment. Taken together, our results reveal multiple roles of Pax6a/b in the control of NC cells during development of the anterior segment.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ia5TgTransgenic Insertion
    ia16TgTransgenic Insertion
      ka96TgTransgenic Insertion
        ka97TgTransgenic Insertion
          ka709
            Small Deletion
            ml4TgTransgenic Insertion
              t3
                Insertion
                tq253a
                  Point Mutation
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  1 - 9 of 9
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                  Fish
                  Antibodies
                  No data available
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  EGFPEFGEGFP
                  EosEFGEos
                  GFPEFGGFP
                  mCherryEFGmCherry
                  1 - 4 of 4
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                  Mapping
                  No data available