PUBLICATION

Stepwise polarisation of developing bilayered epidermis is mediated by aPKC and E-cadherin in zebrafish

Authors
Arora, P., Dongre, S., Raman, R., Sonawane, M.
ID
ZDB-PUB-200123-5
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Sonawane, Mahendra
Keywords
E-cadherin, aPKC, cell biology, cell polarity, developmental biology, epidermis, zebrafish
MeSH Terms
  • Embryo, Nonmammalian
  • Animals
  • Protein Kinase C/analysis
  • Protein Kinase C/metabolism*
  • Cadherins/analysis
  • Cadherins/metabolism*
  • Epidermal Cells/cytology*
  • Epidermal Cells/metabolism
  • Cell Polarity/physiology*
  • Epidermis/embryology*
  • Zebrafish
  • Adherens Junctions/metabolism
(all 12)
PubMed
31967543 Full text @ Elife
Abstract
The epidermis, a multilayered epithelium, surrounds and protects the vertebrate body. It develops from a bilayered epithelium formed of the outer periderm and underlying basal epidermis. How apicobasal polarity is established in the developing epidermis has remained poorly understood. We show that both the periderm and the basal epidermis exhibit polarised distribution of adherens junctions in zebrafish. aPKC, an apical polarity regulator, maintains the robustness of polarisation of E-cadherin- an adherens junction component- in the periderm. E-cadherin in one layer controls the localisation of E-cadherin in the second layer in a layer non-autonomous manner. Importantly, E-cadherin controls the localisation and levels of Lgl, a basolateral polarity regulator, in a layer autonomous as well non-autonomous manner. Since periderm formation from the enveloping layer precedes the formation of the basal epidermis, our analyses suggest that peridermal polarity, initiated by aPKC, is transduced in a stepwise manner by E-cadherin to the basal layer.
Genes / Markers
Figures
Figure Gallery (20 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m567
    Point Mutation
    to6
      Point Mutation
      zf106TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        cdh1MO3-cdh1MRPHLNO
        1 - 1 of 1
        Show
        Fish
        No data available
        Antibodies
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        1 - 1 of 1
        Show
        Mapping
        No data available