PUBLICATION

A morphogenetic EphB/EphrinB code controls hepatopancreatic duct formation

Authors
Thestrup, M.I., Caviglia, S., Cayuso, J., Heyne, R.L.S., Ahmad, R., Hofmeister, W., Satriano, L., Wilkinson, D.G., Andersen, J.B., Ober, E.A.
ID
ZDB-PUB-191121-3
Date
2019
Source
Nature communications   10: 5220 (Journal)
Registered Authors
Caviglia, Sara, Ober, Elke, Wilkinson, David
Keywords
none
MeSH Terms
  • Ephrin-B3/genetics
  • Ephrin-B3/metabolism
  • Bile Ducts/embryology
  • Bile Ducts/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Morphogenesis/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Hepatopancreas/embryology
  • Hepatopancreas/metabolism*
  • Cell Differentiation/genetics
  • Signal Transduction/genetics
  • Receptors, Eph Family/genetics
  • Receptors, Eph Family/metabolism*
  • Animals, Genetically Modified
  • Ligands
  • Protein Binding
  • Ephrin-B1/genetics
  • Ephrin-B1/metabolism*
  • Mutation
  • Gene Expression Profiling
  • Animals
(all 24)
PubMed
31745086 Full text @ Nat. Commun.
Abstract
The hepatopancreatic ductal (HPD) system connects the intrahepatic and intrapancreatic ducts to the intestine and ensures the afferent transport of the bile and pancreatic enzymes. Yet the molecular and cellular mechanisms controlling their differentiation and morphogenesis into a functional ductal system are poorly understood. Here, we characterize HPD system morphogenesis by high-resolution microscopy in zebrafish. The HPD system differentiates from a rod of unpolarized cells into mature ducts by de novo lumen formation in a dynamic multi-step process. The remodeling step from multiple nascent lumina into a single lumen requires active cell intercalation and myosin contractility. We identify key functions for EphB/EphrinB signaling in this dynamic remodeling step. Two EphrinB ligands, EphrinB1 and EphrinB2a, and two EphB receptors, EphB3b and EphB4a, control HPD morphogenesis by remodeling individual ductal compartments, and thereby coordinate the morphogenesis of this multi-compartment ductal system.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fci1TgTransgenic Insertion
    hu3378
      Point Mutation
      hu3393
        Point Mutation
        nim25TgTransgenic Insertion
          nim26
            Indel
            nim27
              Small Deletion
              p314TgTransgenic Insertion
                s854TgTransgenic Insertion
                  1 - 8 of 8
                  Show
                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  ephb3bCRISPR1-ephb3bCRISPR
                  1 - 1 of 1
                  Show
                  Fish
                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  ab1-hnf4apolyclonalIgGGoat
                  Ab1-myl-Ser19-Ppolyclonal
                    Rabbit
                    Ab1-prkczpolyclonalIgGRabbit
                    Ab1-tjp1monoclonalIgG1Mouse
                    Ab2-cdh
                      Ab-2F11monoclonalIgG1Mouse
                      Ab3-cdh
                        Ab5-prox1polyclonalIgGRabbit
                        Ab8-prox1monoclonal
                          IgGMouse
                          1 - 9 of 9
                          Show
                          Orthology
                          No data available
                          Engineered Foreign Genes
                          Marker Marker Type Name
                          CitrineEFGCitrine
                          EGFPEFGEGFP
                          GAL4EFGGAL4
                          GFPEFGGFP
                          1 - 4 of 4
                          Show
                          Mapping
                          No data available