PUBLICATION

Fibroblast Growth Factor Receptors Function Redundantly During Zebrafish Embryonic Development

Authors
Leerberg, D.M., Hopton, R.E., Draper, B.W.
ID
ZDB-PUB-190609-4
Date
2019
Source
Genetics   212(4): 1301-1319 (Journal)
Registered Authors
Draper, Bruce, Leerberg, Dena M.
Keywords
Fibroblast growth factor signaling, midbrain-hindbrain boundary, neurocranium, pectoral fin, posterior mesoderm, viscerocranium
MeSH Terms
  • Brain/embryology
  • Brain/metabolism*
  • Mesoderm/embryology
  • Mesoderm/metabolism*
  • Zebrafish
  • Fibroblast Growth Factors/genetics*
  • Fibroblast Growth Factors/metabolism
  • Animals
  • Receptors, Fibroblast Growth Factor/genetics*
  • Receptors, Fibroblast Growth Factor/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Embryonic Development*
(all 13)
PubMed
31175226 Full text @ Genetics
Abstract
Fibroblast growth factor (Fgf) signaling regulates many processes during development. In most cases, one tissue layer secretes an Fgf ligand that binds and activates an Fgf receptor (Fgfr) expressed by a neighboring tissue. Although studies have identified the roles of specific Fgf ligands during development, less is known about the requirements for the receptors. We have generated null mutations in each of the five fgfr genes in zebrafish. Considering the diverse requirements for Fgf signaling throughout development and that null mutations in the mouse Fgfr1 and Fgfr2 genes are embryonic lethal, it was surprising that all zebrafish homozygous mutants are viable and fertile, with no discernable embryonic defect. Instead, we find that multiple receptors are involved in coordinating most Fgf-dependent developmental processes. For example, mutations in the ligand fgf8a cause loss of the midbrain-hindbrain boundary, whereas in the fgfr mutants, this phenotype is only seen in embryos that are triple mutant for fgfr1a;fgfr1b;fgfr2, but not in any single or double mutant combinations. We show that this apparent fgfr redundancy is also seen during the development of several other tissues, including posterior mesoderm, pectoral fins, viscerocranium, and neurocranium. These data are an essential step towards defining the specific Fgfrs that function with particular Fgf ligands to regulate important developmental processes in zebrafish.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
uc42
    Unknown
    uc50
      Small Deletion
      uc51
        Small Deletion
        uc61
          Insertion
          uc62
            Small Deletion
            uc64
              Small Deletion
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              fgfr1aCRISPR1-fgfr1aCRISPR
              fgfr1bCRISPR1-fgfr1bCRISPR
              fgfr2CRISPR2-fgfr2CRISPR
              fgfr3CRISPR1-fgfr3CRISPR
              1 - 4 of 4
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              Fish
              Antibodies
              No data available
              Orthology
              No data available
              Engineered Foreign Genes
              No data available
              Mapping
              No data available