PUBLICATION

Cxcl12a induces snail1b expression to initiate collective migration and sequential Fgf-dependent neuromast formation in the zebrafish posterior lateral line primordium.

Authors

Neelathi, U.M., Dalle Nogare, D., Chitnis, A.B.

ID

ZDB-PUB-180628-3

Date

2018

Source

Development (Cambridge, England) 145(14): (Journal)

Registered Authors

Chitnis, Ajay

Keywords

Cell adhesion molecule, Collective migration, Cxcl12a, Lateral line primordium, Zebrafish, snail1b

MeSH Terms

Time-Lapse Imaging
Nervous System/cytology
Nervous System/embryology*
Cell Movement/drug effects
Cell Movement/genetics*
Chemokines/metabolism
Chemokine CXCL2/metabolism*
Fibroblast Growth Factors/metabolism*
Wnt Signaling Pathway/drug effects
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/metabolism
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Animals
Morpholinos/pharmacology
Models, Biological
Stem Cells/cytology
Stem Cells/drug effects
Stem Cells/metabolism
Lateral Line System/cytology
Lateral Line System/drug effects
Lateral Line System/embryology*
Lateral Line System/metabolism
Gene Expression Regulation, Developmental/drug effects

(all 26)

PubMed

29945870 Full text @ Development

Abstract

The zebrafish posterior lateral line primordium migrates along a path defined by the chemokine Cxcl12a, periodically depositing neuromasts, to pioneer formation of the zebrafish posterior lateral line system. snail1b, known for its role in promoting cell migration, is expressed in leading cells of the primordium in response to Cxcl12a, whereas its expression in trailing cells is inhibited by Fgf signaling. snail1b knockdown delays initiation of primordium migration. This delay is associated with aberrant expansion of epithelial cell adhesion molecule (epcam) and reduction of cadherin 2 expression in the leading part of the primordium. Co-injection of snail1b morpholino with snail1b mRNA prevents the initial delay in migration and restores normal expression of epcam and cadherin 2 The delay in initiating primordium migration in snail1b morphants is accompanied by a delay in sequential formation of trailing Fgf signaling centers and associated protoneuromasts. This delay is not specifically associated with knockdown of snail1b but also with other manipulations that delay migration of the primordium. These observations reveal an unexpected link between the initiation of collective migration and sequential formation of protoneuromasts in the primordium.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
ackr3b	zf106Tg	control	Prim-5		posterior lateral line primordium anterior region	ISH	Fig. 5
ackr3b	zf106Tg	control	Prim-15		posterior lateral line primordium anterior region	ISH	Fig. 5
ackr3b	zf106Tg	control	Long-pec	Not Detected	posterior lateral line primordium	ISH	Fig. 5
ackr3b	zf106Tg + MO1-snai1b	standard conditions	Long-pec		posterior lateral line primordium anterior region	ISH	Fig. 5
ackr3b	zf106Tg + MO1-snai1b	standard conditions	Prim-15		posterior lateral line primordium anterior region	ISH	Fig. 5
ackr3b	zf106Tg + MO1-snai1b	standard conditions	Prim-5		posterior lateral line primordium anterior region	ISH	Fig. 5
cdh2	zf106Tg	chemical treatment by environment: 1-tert-butyl-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine	Prim-15		posterior lateral line primordium posterior region	ISH	Fig. 4
cdh2	zf106Tg	control	Prim-15		posterior lateral line primordium posterior region	ISH	Fig. 4
cdh2	zf106Tg + MO1-snai1b	standard conditions	Prim-15		posterior lateral line primordium posterior region	ISH	Fig. 4
cxcl12a	zf106Tg	heat shock	Prim-15		horizontal myoseptum	ISH	Fig. 2

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
pd1Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium anterior region etv4 expression decreased amount, abnormal	Fig. 1
pd1Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium anterior region snai1b expression mislocalised, abnormal	Fig. 1
pd1Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium central region etv4 expression decreased amount, abnormal	Fig. 1
pd1Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium central region snai1b expression mislocalised, abnormal	Fig. 1
pd1Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium posterior lateral line development decreased process quality, abnormal	Fig. 1
w32Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium lef1 expression absent, abnormal	Fig. 1
w32Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium anterior region snai1b expression mislocalised, abnormal	Fig. 1
w32Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium central region snai1b expression mislocalised, abnormal	Fig. 1
w32Tg; zf106Tg	heat shock	Prim-15	posterior lateral line primordium posterior lateral line development decreased process quality, abnormal	Fig. 1
zf106Tg	chemical treatment by environment: 6-bromoindirubin-3'-oxime	Prim-15	posterior lateral line primordium anterior region lef1 expression mislocalised, abnormal	Fig. 1

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Mutations / Transgenics

Allele	Construct	Type
pd1Tg	Tg(hsp70l:dnfgfr1a-EGFP)	Transgenic Insertion
w32Tg	Tg(hsp70l:dkk1b-GFP)	Transgenic Insertion
zf51Tg	Tg(hsp70l:cxcl12a)	Transgenic Insertion
zf106Tg	Tg(-8.0cldnb:LY-EGFP)	Transgenic Insertion

1 - 4 of 4

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
ackr3b	MO6-ackr3b	MRPHLNO
cxcl12a	MO4-cxcl12a	MRPHLNO
epcam	MO1-epcam	MRPHLNO
snai1b	MO1-snai1b	MRPHLNO
snai1b	MO4-snai1b	MRPHLNO
tp53	MO4-tp53	MRPHLNO

Fish

Fish
pd1Tg; zf106Tg
w32Tg; zf106Tg
zf106Tg
zf106Tg + MO1-epcam
zf106Tg + MO1-epcam + MO1-snai1b
zf106Tg + MO1-snai1b
zf106Tg + MO4-cxcl12a
zf106Tg + MO4-snai1b
zf106Tg + MO6-ackr3b
zf106Tg; zf51Tg

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GFP	EFG	GFP

Mapping

Neelathi et al., 2018 ZDB-PUB-180628-3 PMID:29945870

Cxcl12a induces snail1b expression to initiate collective migration and sequential Fgf-dependent neuromast formation in the zebrafish posterior lateral line primordium.

Neelathi et al., 2018

ZDB-PUB-180628-3
PMID:29945870