PUBLICATION

Evolutionary emergence of the rac3b/rfng/sgca regulatory cluster refined mechanisms for hindbrain boundaries formation.

Authors
Letelier, J., Terriente, J., Belzunce, I., Voltes, A., Undurraga, C.A., Polvillo, R., Devos, L., Tena, J.J., Maeso, I., Retaux, S., Gomez-Skarmeta, J.L., Martínez-Morales, J.R., Pujades, C.
ID
ZDB-PUB-180404-3
Date
2018
Source
Proceedings of the National Academy of Sciences of the United States of America   115(16): E3731-E3740 (Journal)
Registered Authors
Gómez-Skarmeta, José Luis, Letelier, Joaquin, Maeso, Ignacio, Martinez-Morales, Juan R., Pujades, Cristina, Tena, Juan, Undurraga, Cristian
Keywords
cis-regulatory elements, hindbrain boundaries, regulatory landscape, rhombomeres, segmentation
Datasets
GEO:GSE109219
MeSH Terms
  • CRISPR-Cas Systems
  • Evolution, Molecular
  • Characidae/genetics
  • Characidae/physiology
  • Gene Expression Regulation, Developmental*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Sarcoglycans/genetics
  • Sarcoglycans/physiology*
  • Phylogeny
  • Cell Movement
  • Fishes/classification
  • Fishes/genetics
  • rac GTP-Binding Proteins/genetics
  • rac GTP-Binding Proteins/physiology*
  • Chromatin/genetics
  • Chromatin/ultrastructure
  • Mutagenesis, Site-Directed
  • Actomyosin/physiology*
  • Species Specificity
  • Body Patterning/genetics
  • Neurogenesis
  • Rhombencephalon/embryology*
  • Animals
  • Morphogenesis
  • Zebrafish/embryology*
  • Zebrafish/genetics
(all 27)
PubMed
29610331 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Developmental programs often rely on parallel morphogenetic mechanisms that guarantee precise tissue architecture. While redundancy constitutes an obvious selective advantage, little is known on how novel morphogenetic mechanisms emerge during evolution. In zebrafish, rhombomeric boundaries behave as an elastic barrier, preventing cell intermingling between adjacent compartments. Here, we identify the fundamental role of the small-GTPase Rac3b in actomyosin cable assembly at hindbrain boundaries. We show that the novel rac3b/rfng/sgca regulatory cluster, which is specifically expressed at the boundaries, emerged in the Ostariophysi superorder by chromosomal rearrangement that generated new cis-regulatory interactions. By combining 4C-seq, ATAC-seq, transgenesis, and CRISPR-induced deletions, we characterized this regulatory domain, identifying hindbrain boundary-specific cis-regulatory elements. Our results suggest that the capacity of boundaries to act as an elastic mesh for segregating rhombomeric cells evolved by cooption of critical genes to a novel regulatory block, refining the mechanisms for hindbrain segmentation.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
e1954TgTransgenic Insertion
    e2212TgTransgenic Insertion
      ens1TgTransgenic Insertion
        upo337TgTransgenic Insertion
          upo338TgTransgenic Insertion
            upo339TgTransgenic Insertion
              upo340TgTransgenic Insertion
                upo341TgTransgenic Insertion
                  upo342TgTransgenic Insertion
                    upo343TgTransgenic Insertion
                      1 - 10 of 19
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      1 - 8 of 8
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                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GFPEFGGFP
                      KALTA4EFGKALTA4
                      mCherryEFGmCherry
                      1 - 4 of 4
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                      Mapping
                      No data available